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Lines 4-n must entered in the same order as the slices in the stack. It doesn't matter which one is the reference image. Where fiducial coordinates are unavailable or unreliable, use the number 10000 for both the x and y stage coordinate in the file. Each slice must be represented by the same number of fiducial coordinates in the data file. As you can see, using the text file method introduces many possible sources of errors, so results are not usually as good with this method as with the on-screen method. An example stack with text file fiducial points ('RegistrationExample.bin') is available by FTP from the NIH Image web site in the /pub/nih-image/stacks directory. This routine was written by Michael Castle (mike.castle@umich.edu) of the University of Michigan Mental Health Research Institute (MHRI). |
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Steve Barrett November 2014 |
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