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| File Edit Options Process Analyze Video Macros Stacks SEM SPM Windows Help |
Stack to Windows Add Slice Delete Slice Next Slice > Last Slice < Make Movie Capture Frames Animate Average Make Montage Capture Color RGB to 24-bit Color 8-bit Color to RGB RGB to HSV Register Project Reslice Stack Info Auto Register Depth Of Focus Make Mosaic |
Creates an
image which is a composite of the slices in a stack, calculated
pixel-by-pixel according to how well each part of the image is focused.
Thus, the composite image will compensate for the limited depth of
focus of slices. A dialog box (suppressed if the option key is pressed)
allows the user to optimise the calculation by setting the distances
over which the calculation of the degree of focus is carried out. The
'near' distance should be set to the size of the smallest detail
visible in a well-focussed slice. If the images contain features of a
characteristic size, the 'far' distance should be set to about half of
that size, otherwise it should be set equal to the 'near' distance. To
work properly, the slices in the stack have to be in registry with each
other (see above), as any relative translation or rotation will
produced ghosting (if a weighted average is chosen) or abrupt changes
in intensity (if not). Press the option key to bypass the dialog box.
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Steve Barrett November 2014 |
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