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Several macros (in the file 'Stacks') are available for performing various operations on the slices making up a volume prior to using the Project command. Use 'Smooth' to reduce noise in a volume. Since the projection routine assumes volumes contain one or more objects within a black background, use 'Invert' to invert volumes with a white background. For low contrast volumes, increase the contrast using the controls in the Map window and then apply the look-up table to the volume using the 'Apply LUT' macro. The 'Remove 0 and 255' macro changes pixel values of 0 and 255 to 1 and 254 respectively. This is sometimes useful when pseudocoloring projections. Use 'Replicate Slices' to eliminate the gaps seen in projections of volumes with a slice interval greater than one by increasing the number of slices. The 'Make Cone' macro creates a 643 volume containing a cone with two rods inside that is very useful for understanding the effect of various projection parameters. This routine was written by Michael Castle (e-mail: mike.castle@umich.edu) and Janice Keller of the University of Michigan Mental Health Research Institute (MHRI). |
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Steve Barrett November 2014 |
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