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Specialist Analysis >

NIH Image

Computes the area and mean gray value of the current selection (or length if it's a line selection) and displays the results in the Info and Results windows. Use the Options dialog box to enable other measurements, such as perimeter. Use Undo to delete the last measurement or Delete Measurement to delete an arbitrary measurement. Only highlighted pixels are included in the computation if either density slicing or thresholding are enabled. The measurement counter is incremented by one each time you use Measure. Use Reset to reset it to zero. The default maximum number of measurements is 200, but this can be increased in the Options dialog box. Use Show Results to display the list of measurement results, Print (with either the Info or Results window active) to print the results, Copy to copy results to the Clipboard, and Export to export results to a tab-delimited (spreadsheet compatible) text file. There is a 32K byte limit when displaying, printing, or copying results, but no limit to the number of measurements that can be exported to a text file. Calculated areas are displayed in calibrated units, such as square millimeters, if the Set Scale command has been used to establish the spatial scale. Density readings are displayed in calibrated units, such as optical density or isotope concentration, if the Calibrate command has been used to perform density calibration. The most efficient way to organize measurement results is to have a spreadsheet loaded at the same time you are using NIH Image. Make a series of measurements (cmd-1), Copy them to the Clipboard (cmd-C), click on the spreadsheet to activate it, select the cell where you want the measurements stored, Paste the results (cmd-V), click on the image window to reactivate NIH Image, and finally, Reset the measurement counter.



Steve Barrett     November 2014


S.D.Barrett @ liv.ac.uk