Melanoma Cell Lines

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I.D.	Description	Origin	Comments	Location	Cell Lysate Bank
A2058	Melanoma Cells, Ras wt, BRAF V600E	Richard Marais
A375P (low invasive)	Melanoma Cells, Ras wt, BRAF V600E, PTEN wt	Richard Marais	DMEM + Glutamax FBS 10% NEAA 1% Penicillin/streptomycin 1%	Cryostore	P2+P3 Box3 24/11/15 Code: A37 >120 aliquots 1.2µg/µl
501MEL (pigmented)	Melanoma Cells, N-Ras wt, BRAF V600E, PTEN mut	Richard Marais	DMEM + Glutamax FBS 10% NEAA 1% Penicillin/streptomycin 1%	Cryostore	P2+P3 Box2 24/11/15 Code: 50M >120 aliquots 1.2µg/µl
WM266.4 (highly invasive)	Melanoma Cells, Ras wt, BRAF V600D, PTEN null	Richard Marais	DMEM + Glutamax FBS 10% NEAA 1% Penicillin/streptomycin 1% Max passage P30 Split every 2-3 days, 1 in 10 or 1 in 5	Cryostore	P2+P2 Box2 20/08/15 Code: WM2 89 aliquots 0.912µg/µl
MNT1 (very pigmented)	Melanoma Cells, Ras wt, BRAF V600E , PTEN wt	Graca Raposo	DMEM + Glutamax AIM-V 10% FBS 20% NEAA 1% Sodium pyruvate 1% Penicillin/streptomycin 1% Max passage P3 + P10 Split every 2-3 days, 1 in 10 or 1 in 5	Cryostore	P3+P5 Box2 20/08/15 Code: MNT 113 aliquots 0.76µg/µl
Melan-a (pigmented)	Non-tumorigenic immortalized mouse melanocytes	Elena Sviderskaya Contact to buy the cells: Philip Goff Wellcome Trust Functional Genomics Cell Bank	10% CO2 incubator RPMI 1640 + Glutamax FBS 10% Penicillin/streptomycin 1% Mix RPMI + P/S, let ON in the incubator (cap unscrewed), then add the FBS the next day TPA 200nM (always added fresh on the cells) Only after thawing the cells: Add PTU at 200 µM, After 2-3 days change with medium without PTU, and let the cells grow and become darker for a least a week. Max passage P20 + P10 Split every 2-3 days, 2 in 5 or 1 in 2	Cryostore