NIH Image

Enables and disables density slicing. Like thresholding, density slicing allows objects to segmented on the bases of gray level. When density slicing is enabled, objects are highlighted in red and background pixels are left unchanged. As in thresholding, background pixels are ignored when making area and density measurements. Click and drag in the LUT window using the LUT tool to vary the size and location of the density slice. As you manipulate the density slice, its upper and lower limits are continuously displayed in the Info window.

In the LUT window, you can double click within the density slice using the eyedropper tool to change the highlighting color to something other than the default red. As a shortcut, you can enable density slicing by double clicking on either the wand or LUT tools.

To prevent desktop items, such as the menu bar, from changing color, white (0) and black (255) entries in the LUT are never included within the density slice. Therefore, thresholding usually works better for discriminating objects containing black pixels.

Use the Apply LUT command to set all pixels which are within the density slice to the foreground color (or leave then unchanged) and all other pixels to the background color (or leave them unchanged). The Make Binary command will set pixels within the density slice to black, and all other pixels to white.