2024
McCarron,K.R., Elcocks,H., Mortiboys,H., Urbé,S. and Clague,M.J. (2024) The Parkinson’s Disease related mutant VPS35 (D620N) amplifies LRRK2 response to endolysosomal stress. Biochem J. 481:265-278 link.
Glover,H.L., Mendes,M., Gomes-Neto,J., Rusilowicz-Jones,E., Rigden,D., Dittmar,G., Urbé,S., Clague,M.J. (2024) Microtubule association of TRIM3 revealed by differential extraction proteomics J. Cell Sci. 137:jcs261522 link.
2023
Tey P-Y., Dufner A., Knobeloch, K-P., Pruneda,J.N., Clague, M.J. and Urbé,S. (2023) Rapid turnover of CTLA4 is associated with a complex architecture of reversible ubiquitylation. bioRxiv link
Barone, F.G., Marcello,M., Urbé,S., Sanchez-Soriano, N. and Clague,M.J. (2023) Whole organism and tissue specific analysis of pexophagy in Drosophila bioRxiv link
Clancy,A., Rusilowicz-Jones,E.V., Wallace,I., Swatek,K.N., Urbé,S. And Clague,M.J. (2023) ISGylation-independent protection of cell growth by USP18 following interferon stimulation. Biochem J. 480, 1571-1581 link
Barone,F.G., Urbé,S. and Clague,M.J. (2023) Segregation of pathways leading to pexophagy. Life Science Alliance, DOI: 10.26508/lsa.2022018 link
Elcocks,H., Brazel,A.J., McCarron,K., Kaulich,M., Husniak,K., Mortiboys,H., Clague,M.J. and Urbé,S. (2023) FBXL4 deficiency promotes mitophagy by elevating NIX. EMBO J., 42: e112799 link.
Using an E3 ubiquitin ligase CRISPR/Cas9 screen, we identify FBXL4 and VHL as the major suppressors of mitophagy. We show that these converge on the regulation of NIX expression through different mechanisms. FBXL4 regulates NIX stability and this is likely the major defect associated with FBXL4 mutations leading to mitochondrial depletion syndrome.
2022
Bertsoulaki, E., Glover,H.L., Gomes-Neto, J.I., Pizer,B., Maiato, H., Urbé,S. and Clague,M.J. (2022) The deubiquitylase USP31 controls the Chromosomal Passenger Complex and spindle dynamics. bioRxiv link.
Rusilowicz-Jones,E.V., Urbé,S. and Clague,M.J. (2022) Protein degradation on the global scale. Molecular Cell, 82, 1414-1423 link.
Rusilowicz-Jones,E.V., Brazel,A., Frigenti,F., Urbé,S. and Clague,M.J. (2022) Sub-cellular mapping of the ubiquitin system. Seminars in Cell and Developmental Biology, S1084-9521(21)00292-5 link.
Rusilowicz-Jones,E.V, Barone,F.G., Lopes Martins,F., Stephen,E., Mortiboys, H., Urbé,S. and Clague,M.J. (2022) Benchmarking a highly selective USP30 inhibitor for enhancement of mitophagy and pexophagy. Life Science Alliance, e202101287 DOI: 10.26508/lsa.202101287 link.
2021
Pollock,L., Jardine,J., Urbé,S. and Clague,M.J. (2021) The PINK1 repertoire: not just a one trick pony. Bioessays, 43(11):e2100168 link.
Clancy,A., Heride,C. Pinto-Fernández,A., Elcocks,H., Kallinos,A., Kayser-Bricker,K.J., Wang, W., Smith,V. Davis,S., Fessler,S., McKinnon,C., Katz,M., Hammonds,T., Jones, N.P., O’Connell,J., Follows,B., Mischke, S., Caravella,J.A., Ioannidis,S., Dinsmore,C., Kim, S., Behrens,A., Komander,D., Kessler,B.M., Urbé,S, Clague,M.J. (2021) The deubiquitylase USP9X controls ribosomal stalling. J. Cell Biol., 220 (3): e202004211 link.
We introduce the first selective small molecule inhibitor of USP9X and use this to open up new biology linking USP9X with ribosomal quality control.
Ruiz,E.J., Pinto-Fernandez,A., Turnbull,A.P., Lan,L., Charlton,T.M., Scott,H.C., Damianou,A., Vere,G., Riising,E.M., Da Costa,C., Krajewski,W.W., Guerin,D. Kerns,J., Ioannidis, S., Kayser-Bricker,K., Moncaut,N., Rosewell,I., Saur,D., Nye,E., Jones,N.P., Heride,C., Gersch, M., Dinsmore,C.J., Hammonds,T.R., Kim,S., Komander,D., Urbé,S., Clague,M.J., Kessler,B.M. and Behrens,A. (2021) USP28 deletion and small molecule inhibition destabilises c-Myc and elicits regression of squamous cell lung carcinoma eLife 10:e71596 link.
2020
Rusilowicz-Jones,E., Jardine,J., Kallinos,A., Pinto-Fernandez,A., Guenther,F. Giurrandino,M., Barone,F.G., McCarron,K., Burke,C.J., Murad,A., Martinez,A., Marcassa,E., Gersch,M., Kayser-Bricker,K.J., Buckmelter,A., Lamoliatte,F., Gajbhiye,A., Davis,S., Scott,H.C., Murphy,E., England,K., Mortiboys,H., Komander,D., Trost, M., Kessler,B.M., Ioannidis,S., Ahlijanian,M., Urbé,S., Clague,M.J. (2020) USP30 sets a trigger threshold for PINK1-PARKIN amplification of mitochondrial ubiquitylation. Life Science Alliance, DOI: 10.26508/lsa.202000768 link.
Clague, M.J. and Urbé,S. (2020) Data mining for traffic information. 20th anniversary special issue of Traffic, 21, 162-168 link.
2019
Marcassa,E., Kallinos,A., Jardine,J., Rusilowicz-Jones,E.V., Clague,M.J. and Urbé,S. New aspects of USP30 biology in the regulation of pexophagy. (2019) Autophagy, 9, 1634-1637 link
Clague,M.J., Urbé,S. and Komander,D. (2019) Breaking the chains: deubiquitylating enzyme specificity begets function. Nature Reviews in Molecular Cell Biology, 20, 338-352 link
2018
Pruneda,J.N. Bastidas,R.J., Bertsoulaki,E., Dolat,L. Swatek,K.N., Santhanam,B. Clague,M.J., Valdivia,R.H., Urbé,S. and Komander,D. (2018) A chlamydia effector combining deubiquitination and acetylation activities induces Golgi fragmentation. Nature Microbiology, 3, 1377-1384 link
Marcassa,E., Kallinos,A., Jardine,J., Rusilowicz-Jones,E.V., Martinez,A., Kuel,S., Islinger,M., Clague,M.J. and Urbé,S. Dual role of USP30 in controlling basal pexophagy and mitophagy.(2018) EMBO Reports, 19, e45595 link.
USP30 is a DUB of interest in the context of Parkinsons Disease (PD). Here we show that its depletion elevates basal mitophagy in a manner that requires the PD associated kinase PINK1. We also show localisation to peroxisomes and a parallel regulation of pexophagy that is PINK1 independent. This work was covered in a Current Biology dispatch article.
MacDonald,E., Brown,L., Selvais,A., Liu,H., Waring, T., Newman,D.T., Bithell,J., Grimes,D., Urbé,S., Clague,M.J. and Zech,T. HRS-WASH axis governs actin mediated endosomal recycling and cell invasion. (2018) J. Cell Biol. 217, 2549-2564 link.
Lee,J.J., Sanchez-Martinez,A., Martinez,A., Beninca,C., Mayor,U., Clague,M.J. and Whitworth,A.J. (2018) Basal mitophagy is widespread in Drosophila but only minimally affected by loss of Pink1 or Parkin.
Introduces fly models incorporating reporter systems which allow visualisation of mitophagy. PINK1 and Parkin mutations associated with Parkinsons disease have been linked to defective mitophagy following acute depolarisation. Here we see no changes in basal mitophagy associated with such mutations, despite the fact the flies exhibit characteristic movement disorder.
Fielding,A.J., Concannon,M., Darling,S., Rusilowicz-Jones,E., Sacco,J., Prior,I.A., Clague,M.J., Urbé,S. and Coulson,J.M. (2018) The deubiquitylase USP15 regulates topoisomerase II alpha to maintain genome integrity. Oncogene,37, 2326-2342 link.
2017
Turnbull,A.P., Ioannidis,S., Krajewski,W., Pinto-Fernandez,A., Heride,C. Martin,A.C.L., Tonkin,L.M., Townsend,L., Buker,S.M.,., Lancia,D.R., Caravella,J.A., Toms,A.V., Charlton, T., Lahdenranta,J., Wilker,E., Follows,B.C., Evans,N.J., Stead,L., Alli,C., Zarayskiy,V.V., Talbot,A.C., Buckmelter,A.J., Wang,M., McKinnon, C.L., Saab,F. McGouran,J.F., Century,H., Gersch,M., Pittman,M.S., Marshall,C.G. Raynham,T.M., Simcox,M., Stewart,L.M.D., McGloughlin,S.B., Escobedo,J.A., Bair,K.W., Dinsmore,C.J., Hammonds,T.R., Kim,S., Urbé,S., Clague,M.J., Kessler,B., Komander,D. (2017) Molecular basis of USP7 inhibition by selective small molecule inhibitors. Nature, 550, 481-486. link
Provides the first description of highly selective DUB inhibitors accompanied by a structural explanation for how selectivity is achieved. Inhibitors of USP7 influence the p53/MDM2 axis and retard tumour growth. The paper was highlighted in Science Signaling and in Cancer Discovery.
Martinez,A., Mayor,U. and Clague,M.J. (2017) Multi-story Parkin. Oncotarget, 8:50327-50328 link
Martinez,A., Lectez,B., Ramirez,J., Popp,O., Sutherland,J.D., Urbé,S., Dittmar,G., Clague,M.J., Mayor,U. (2017) Quantitative proteomic analysis of Parkin substrates in Drosophila neurons. Mol. Neurodegeneration, 12(1):29 link
Describes the identification of substrates of the Parkinson's Disease related protein, Parkin, in fly brains without any artificial induced activation such as acute mitochondrial depolarisation.
Clague,M.J. and Urbé,S. (2017) Integration of cellular ubiquitin and membrane traffic systems: focus on deubiquitylases. FEBS J., 284:1753-1766 link.
2016
Heride,C., Rigden,D.J., Bertsoulaki, E., Cucchi,D., De Smaele, E., Clague,M.J. and Urbé, S. (2016) The centrosomal deubiquitylase, USP21, regulates Gli1 transcriptional activity and stability. J. Cell Sci., 129, 4001-4013 link
Wymant,J.M., Hiscox,S., Westwell,A.D., Urbé S., Clague, M.J., Jones, A.T. (2016) The role of BCA2 in endocytic trafdocytic trafficking of EGFR and significance as a prognostic biomarker in cancer, J. Cancer 9, 2388-2407 link.
Clague,M.J. and Rochin,L. (2016) Parkinsons disease: a traffic jam? Current Biology, 26, R332-R334 link.
2015
Malec,V., Coulson,J.M., Urbé,S. and Clague,M.J. (2015) Combined analyses of the VHL and hypoxia signalling axes in an isogenic pairing of renal clear cell carcinoma cells. J. Proteome Research 14, 5263-72,[abstract].
Mageean,C., Griffiths,J.R., Smith,D.L., Clague,M.J. and Prior,I.A. (2015) Absolute quantification of endogenous RAS isoform abundance. PLoS One, 10:e0142674 [abstract].
Clague,M.J., Heride,C. and Urbé,S. (2015) The demographics of the ubiquitin system. Trends in Cell Biology, 25, 417-426 [online].
Sacco,J., Kenyani, J., Butt,Z., Carter,R., Chew,H.Y., Cheeseman, L.P, Darling,S., Denny,M., Urbé,S., Clague,M.J. and Coulson, J.M. Loss of the deubiquitylase BAP1 alters class I histone deacetylase expression and sensitivity of mesothelioma cells to HDAC inhibitors. Oncotarget, 6, 13757-13771[abstract].
Liang, J-R., Martinez,A., Mayor,U., Lane,J., Clague,M.J. and Urbé,S. (2015) USP30 deubiquitylates mitochondrial Parkin substrates and restricts apoptotic cell death. EMBO Reports, 16, 618-627 [abstract].
Sacco, J.and Clague,M.J. (2015) Dysregulation of the Met pathway in lung cancer: implications for drug targeting and resistance, Translational Lung Cancer Research, 4, 242-252 [abstract].
Hammond,D.E., Mageean,C.J., Rusilowicz,E.V., Wickenden,J.A., Clague,M.J. and Prior,I.A. (2015) Differential reprogramming of isogenic colorectal cancer cells by distinct activating KRAS mutations. J. Proteome Research, 14, 1535-1546 [abstract].
2014
Tang,W.Y.Y., Beckett,A.J., Prior,I.A., Coulson,J.M., Urbé,S. and Clague,M.J. (2014) Plasticity of mammary cell boundaries governed by EGF and actin remodeling Cell Reports, 8, 1722-1730 [open access]
Nishi,R., Wijnhoven,P., le Sage,C., Tjeertes,J., Galanty,Y., Forment,J.V., Clague,M.J., Urbé,S. and Jackson,S.P. (2014) Systematic characterization of deubiquitylating enzymes for roles in maintaining genome integrity Nature Cell Biology, 16, 1016-1026 [paper]
Musiani,D., Hammond,D.E., Cirillo,L., Erriquez,J., Olivero,M., Clague,M.J. and Di Renzo,M.F. (2014) PIM2 kinase is induced by cisplatin in ovarian cancer cells and limits drug effectiveness. J. Proteome Research, 11, 4970-4982 [paper].
MacDonald, E. Urbé,S. and Clague,M.J. (2014) USP8 controls the trafficking and sorting of lysosomal enzymes. Traffic, 15, 879-888 [abstract].
Heride,C., Urbé, S. and Clague,M.J. (2014) Ubiquitin code assembly and disassembly. Current Biology, 24, R215-R220 [abstract].
Sacco,J., Yau,T.J., Darling,S. Patel,V., Liu,H., Urbé,S., Clague,M.J. and Coulson,J.M. (2014) The deubiquitylase ATXN3 restricts PTEN transcription in lung cancer cells, Oncogene, 33, 4265-4272 [abstract].
2013
Clague,M.J., Barsukov,I., Coulson,J.M., Liu,H., Rigden,D.J. and Urbé,S. (2013) Deubiquitylases from genes to organism. Physiological Reviews 93, 1289-1315 [abstract]
Faronato,M., Patel,V., Darling,S., Dearden,L., Clague,M.J., Urbé, S. and Coulson,J.M. (2013) The deubiquitylase USP15 stabilises newly synthesised REST and rescues its expression at mitotic exit. Cell Cycle, 12, 1964-1977 [abstract]
Clague,M.J. (2013) Oxidation controls the DUB step. Nature 497, 49-50 [article]
Liu,H., Urbé,S. and Clague, M.J. (2013) Regulation of endocytic trafficking and signaling by deubiquitylating enzymes. Vesicle Trafficking in Cancer eds. Yarden.Y. and Tarcic, G. Springer press. 245-259.[abstract]
2012
Hayes,S.D., Liu,H., MacDonald,E., Sanderson,C.M., Coulson,J.M., Clague,M.J. and Urbé,S. Direct and indirect control of MAPK pathway associated components, BRAP/IMP and CRAF, by the deubiquitylating enzyme USP15. J. Biol. Chem. 287, 43007-43018 [abstract].
Omerovic,J., Hammond,D.E., Prior,I.A. and Clague,M.J. (2012) A global snap-shot of the influence of endocytosis upon EGF receptor signaling output. Journal of Proteome Research 11, 5157-5166 [abstract].
Clague,M.J., Liu,H. and Urbé,S. (2012) Governance of endocytic trafficking and signaling by reversible ubiquitylation. Dev. Cell 23, 457-467 [abstract].
Urbé,S., Liu,H., Hayes,S.D., Heride,C., Rigden,D.J. and Clague,M.J. (2012) Systematic survey of deubiquitinase localisation identifies USP21 as a regulator of centrosome and microtubule associated functions. Mol. Biol. Cell 23, 1095-1103 [abstract].
Liu,H., Urbé,S. and Clague,M.J. (2012) Selective protein degradation in cell signaling. Seminars in Cell and Developmental Biology23, 509-514 [abstract].
Clague,M.J., Coulson,J.M. and Urbé,S. (2012) Cellular functions of the DUBs. J. Cell Sci. 125,277-286 [abstract].
2011
Elliot,P.R., Liu,H., Pastok,M.Y., Grossman,G.J., Rigden,D., Clague,M.J., Urbé,S. and Barsukov, I. (2011) Structural variability of the Ubiquitin Specific Protease DUSP-UBL double domains. FEBS Lett. 585, 3385-3390 [abstract].
Clague,M.J. (2011) Met receptor: a moving target Science Signaling 4, pe40 [abstract].
Thorne, C.T., Eccles, R.L., Coulson,J.M., Urbé,S. and Clague,M.J. (2011) Isoform specific localisation of the deubiquitinase USP33 to the Golgi apparatus, Traffic 12, 1563-1574 [abstract].
2010
Clague,M.J. and Urbé,S. (2010) Ubiquitin: same molecule, different degradation pathways. Cell, 143, 682-685 [abstract]
Polson, H.E.J., De Lartigue,J., Rigden,D.J., Reedijk,M., Urbé,S., Clague,M.J., Tooze,S.A. (2010) Mammalian Atg18 (WIPI2) localises to omegasome-anchored isolation membranes and positively regulates Lulates LC3 lipidation Autophagy, 4, 506-522 [abstract].
Hammond,D.E., Hyde, R., Kratchmarova,I., Beynon,R.J., Blagoev,B. and Clague,M.J. (2010) Quantitative analysis of HGF and EGF-dependent phosphotyrosine signalling networks. Journal of Proteome Research 7, 2734-2742 [abstract].
Omerovic, J., Clague,M.J. and Prior,I.A. (2010) Phosphatome profiling reveals PTPN2, PTPRJ and PTEN as negative regulators of PKB/Akt activation in Ras mutated cancer cells. Biochem J. 426, 65-72 [abstract]
Sacco, J.J., Coulson,J.M., Clague,M.J. and Urbé,S. (2010) Emerging roles of deubiquitinases in cancer-associated pathways. IUBMB Life, 62,62, 150-157 [abstract].
2009
Buus, R., Faronato,M., Hammond, D.E., Urbé,S. and Clague M.J. (2009) Deubiquitinase activities required for Hepatocyte Growth Factor-induced scattering of epithelial cells Current Biology 19, 1463-1466 [article]
Komander, D.,Clague,M.J. and Urbé,S. (2009) Breaking the chains: structure and function of the deubiquitinases. Nature Reviews of Molecular Cell Biology 10, 550-563 [article].
Liu, H., Buus, R. Clague, M.J. and Urbé, S. (2009) Regulation of ErbB2 status by the proteasomal DUB POH1. PLoS One, 4 (5): e5544 [article].
De Lartigue, J., Polson,H., Feldman, M., Shokat, K., Tooze, S.A., Urbé, S. and Clague, M.J. (2009) PIKfyve regulation of endosome-linked pathways. Traffic, 10, 883-893 [article].
Rigden,D.J., Liu, H., Hayes,S.D., Urbé, S. and Clague, M.J. (2009) Ab initio protein modelling reveals novel human MIT domains. FEBS Lett. 583, 872-878 [article].
Clague,M.J.,Urbé, S. and De Lartigue,J. (2009) Phosphoinositides and the endocytic pathway. Experimental Cell Research, 9, 1627-1631. [article]
Doherty, M.K, Hammond, D.E., Clague,M.J., Gaskell, S.J. and Beynon, R.J. (2009) Turnover of the human proteome: determination of intracellular protein stability by dynamic SILAC. Journal of Proteome Research 8, 104-112 [article].
2008
Clague,M.J. and Urbé, S. (2008) Quick guide: Multivesicular bodies Current Biology 18, R402-R404 [article]
Clague, M.J. Coulson,J.M. and Urbé, S. (2008) Deciphering histone H2A deubiquitination. Genome Biology 9, 202 [article]
Omerovic, J., Hammond, D.E., Clague,M.J. and Prior, I.A. (2008) Ras isoform abundance and signalling in human cancer cell lines. Oncogene,27, 2754-2762 [article].
2007.
Row,PE, Liu,H,, Hayes,S. Welchman,R., Charalabous,P., Hofmann,K., Clague,MJ., Sanderson,CM. and Urbé,S. (2007)The MIT domain of UBPY constitutes a CHMP binding and endosomal localisation signal required for efficient EGF receptor degradation. J. Biol. Chem. 282, 30929-37 [abstract].
Ross SH, Lindsay Y, Safrany ST, Lorenzo O, Villa F, Toth R, Clague MJ, Downes CP, Leslie NR. (2007) Differential redox regulation within the PTP superfamily. Cell Signal. 19, 1521-30.
2006
Clague, M.J and Urbé, S. (2006) Endocytosis: the DUB version. Trends in Cell Biology 16, 551-559 [abstract]
Lorenzo, O., Urbé,S. and Clague,M.J. (2006) Systematic analysis of myotubularins: heteromeric interactions, subcellular localisation and endosome related functions. J. Cell Sci. 119, 2953-2959 [abstract].
Hammond, D.E. and Clague,M.J. (2006) Membrane Traffic: Catching the lysosome express. Current Biology, 16, R416-R418 [abstract].
Row P.E., Prior,I.A., McCullough J., Clague M.J., Urbé S. (2006) The ubiquitin isopeptidase UBPY regulates endosomal ubiquitin dynamics and is essential for receptor down-regulation. J. Biol. Chem. 281, 12618-12624 [abstract].
McCullough J., Row P.E., Lorenzo, O. Doherty M., Beynon R., Clague M.J., Urbé S. (2006) Activation of the endosome associated deubiquitinating enzyme AMSH by STAM, a component of the multivesicular body pathway.Current Biology, 16, 160-165 [abstract].
2005
Clague,M.J. and Lorenzo,O. (2005) The myotubularin family of lipid phosphatases. Traffic 6, 1033-1069 [abstract].
Row,P.E., Clague,M.J. and Urbé,S. (2005) Gr (2005) Growth factors induce differential phosphorylation profiles of the Hrs-STAM complex: a common node in signalling networks with signal-specific properties.Biochem. J. 389, 629-636 [article].
Lorenzo,O., Urbé,S. and Clague,M.J. (2005) Analysis of phosphoinositide binding domain function in myotubularin related protein 3, MTMR3. J. Cell Sci. 118, 2005-2012 [abstract].
2004
Carter,S., Urbé,S. and Clague,M.J. (2004) The Met receptor degradation pathway: requirement for K48-linked polyubiquitin independent of proteasome activity. J. Biol. Chem. 51, 52835-52839 [abstract].
McCullough,J., Clague,M.J. and Urbé,S. (2004) AMSH: an endosome associated ubiquitin isopeptidase. J. Cell Biol. 166, 487-492 [abstract].
Hammond,D.E., Carter,S. and Clague,M.J. (20 (2004) Met receptor dynamics and signalling. Current Topics in Microbiology and Immunology 286, 21-44.
Clague,M.J., Dove.S.K. and Barr,F.A. (2004) I-proteins: a proposed switch in myoyotubularin function. Trends in Biochemical Sciences 29,58-61 [abstract].
2003
Clague,M.J. and Urbé,S. (2003) Hrs function: viruses provide the clue. Trends in Cell Biology 13, 603-606 [abstract].
Urbé,S., Sachse,M., Row,P.E., Preisinger,C., Barr,F.A., Strous,G., Klumperman,J. and Clague,M.J. (2003) The UIM domain of Hrs couples receptor sorting to vesicle formation. J. Cell Sci. 116, 4169-4179 [abstract].
Hammond,D.E. Carter,S., McCullough,J. Urbé,S, Vande Woude,G. and Clague,M.J. (2003) Endosomal dynamics determine Met signalling. Mol. Biol. Cell 14, 1346-1354 [abstract].
Schaletzky,J., Dove,S.K., Short,B., Lorenzo,O., Clague,M.J. and Barr,F.A. (2003) Phosphatidylinositol-5-phosphate activation and conserved substrate specificity of the myotubularin family of phosphatidylinositol 3-phosphatases Current Biology 13, 504-509 [article].
Blumenthal,R. Clague,M.J., Durrell, S.R. and Epand,R.M. (2003) Membrane fusion. Chemical Reviews 103, 53-70 [article].
2002
Clague,M.J. (2002) Membrane transport: A coat for ubiquitin. Current Biology, 12, R529-R531 [article].
2001
Walker,D.M., Urbé,S., Dove,S.K., Tenza,D., Raposo,G. and Clague,M.J. (2001) Characterization of MTMR3: an inositol lipid 3-phosphatase with novel substrate specificity. Current Biology 11, 1600-1605. [abstract]
Clague,M.J. and Urbé,S. (2001) The interface of receptor trafficking and signaling, J. Cell Sci.,114, 3075-3081. [abstract]
Hammond,D.E., Urbé,S., Vande Woude,G.F. and Clague,M.J. (2001) Down-regulation of Met, the receptor for hepatocyte growth factor. Oncogene, 20, 2761-2770.[abstract]
Mills,I.G., Urbé,S. and Clague,M.J. (2001) Relationships between EEA1 binding partners and their role in endosome fusion. J. Cell Sci., 114, 1959-1965 [full article PDF]
2000
Clague,M.J. and Herrmann,A. (2000) Membrane transport: deciphering fusion. Current Biology, 10, R750-752.
Urbé,S., Mills,I.G., Stenmark,H., Kitamura,N. and Clague,M.J. (2000) Endosomal localisation and receptor dynamics determine tyrosine phosphorylation of Hrs. Molecular and Cellular Biology, 20, 7685-7692.[abstract] [full article PDF]
Prior,I.A. and Clague,M.J. (2000) Detection of thiol modification following generation of reactive nitrogen species: analysis of synaptic vesicle proteins Biochim. Biophys. Acta, 1475, 273-280 [full article PDF]
Earlier Stuff
Prior IA, Clague MJ.(1999) Localization of a class II phosphatidylinositol 3-kinase, PI3KC2a, to clathrin-coated vesicles . Mol Cell Biol Res Commun. 1, 162-166. [full article pdf]
Clague, M.J. (1998) Molecular aspects of the endocytic pathway. Biochem. J. 336, 271-282 [full text]
Mills,I.G., Jones,A.T. and Clague,M.J. (1998) Involvement of the endosomal autoantigen EEA1 in homotypic fusion of early endosomes. Current Biology, 8, 881-884. [abstract]
Jones,A.T., Mills,I.G., Scheidig,A.J., Alexandrov,K. and Clague,M.J. (1998) Inhibition of endosome fusion by wortmannin persists in the presence of activated rab5. Mol. Biol. Cell 9, 323-332. [full text]
Nüßler,F., Clague,M.J., Herrmann,A. (1997) Meta-stability of the hemifusion intermediate induced by glycosylphosphatidylinositol-anchored influenza hemagglutinin. Biophys. J. 73, 2280-2291 [abstract]
Gaffet,P., Jones,A.T. and Clague,M.J. (1997) Inhibition of cation-independent mannose 6-phosphate receptor incorporation into trans-Golgi nans-Golgi network-derived clathrin-coated vesicles by wortmannin. J. Biol. Chem. 272, 24170-24175 [abstract]
Prior, I.A. & Clague,M.J. (1997) Glutamate uptake occurs at an early stage of synaptic vesicle recycling. Current Biology 7, 353-356 [abstract]
Jones,A.T. and Clague,M.J. (1995) Phosphatidylinositol 3-kinase activity is required for early endosome fusion. Biochem J. 311, 31-34. [abstrract]
Clague,M.J., Thorpe,C. & Jones,A.T. (1995) Phosphatidylinositol 3-k-kinase regulation of fluid phase endocytosis FEBS Lett. 367, 272-274.
Clague,M.J., Urbé,S., Aniento,F., & Gruenberg.J. (1994). Vacuolar-ATPase activity is required for endosomal carrier vesicle formation. J.Biol.Chem. 269, 21-24. [pdf]