Image SXM Help

NIH Image

Saves (in a window named "Blank Field") a background image that will be used to correct for uneven illumination. The Stop Capturing command will use this reference image to correct shading errors in newly acquired images. It does this by dividing each pixel in the newly acquired image by a correction factor computed for each pixel in the blank field. This correction factor is generated by dividing each gray value in the blank field by the mean gray value. You can capture a single frame without shading correction by holding down the option key while selecting Stop Capturing. Close the "Blank Field" window to stop doing shading correction.