MTMR7

ACCESSION CR749240

from RZPD

cDNA sequence:

gggcgcccgcccgggaccctgcagacgtgggccagccatggagcacatcc
gcacgcccaaggttgaaaatgtccgcttggtagatcgagtgtctcctaaa
aaagcagctctaggtactttgtatttgacggctacccatgtcatattcgt
ggaaaattcacctgacgcaagaaaagaaacatggattcttcacagtcaga
tttccaccattgagaaacaggcaacaaccgctaccggatgccctctgctg
attcgctgcaagaactttcagataatacagctcatcatacctcaggaaag
agattgccacgacgtgtacatctccctgatacgccttgcaaggccagtga
aatatgaggagttatactgcttttcattcaaccccatgctggataaagaa
gaaagagagcaaggctgggtgctgatcgatcttagtgaagaatacacgcg
gatgggcctccctaatcattactggcagctcagcgatgtgaatagagact
acagagtctgtgactcttatcctactgaactgtacgttcccaaatcggcc
acggcacacatcatagtggggagttccaaattccggagtagacggcgatt
tcctgtcctttcttactattataaagataaccacgcctccatctgccgga
gcagccagcccctgtccggcttcagtgcccggtgcctggaggacgagcag
atgctccaggccattaggaaagccaatccaggaagtgacttcgtttatgt
cgttgacgcccggcctaaacttaatgcaatggcaaatcgtgctgcaggga
aaggctatgagaatgaagacaattattccaatatcaagtttcagtttatc
gggatagagaacatccatgtcatgaggaacagtctgcagaaaatgctgga
agtgtgtgaacttaaatctccctccatgagtgatttcctgtggggtctgg
agaactctggctggttaaggcacattaaagccataatggatgcaggaatc
ttcattgcaaaggcagtgtcagaggaaggggcaagtgtgcttgttcactg
ttctgatggctgggacaggaccgctcaggtgtgctcggtggcaagcctgc
tgctggaccctcactaccggactctgaagggcttcatggtattaattgaa
aaggactggatttcctttggtcataagtttaatcaccgatatggcaatct
agatggtgacccaaaagaaatctctccagttattgaccagttcattgagt
gtgtttggcagttaatggaacaatttccctgtgcctttgagttcaatgag
aggtttttgattcacattcaacatcacatttattcctgccagtttggaaa
cttcctatgtaacagccaaaaggagagacgagaactcaagattcaagaaa
gaacatactcattatgggctcacctgtggaagaatcgggccgactacctg
aatcctctgtttagagctgatcacagccagactcagggaacccttcatct
ccctacaacaccatgtaacttcatgtacaagttttggagtggaatgtata
accgctttgaaaaggggatgcagccccgacagtcagttacagattaccta
atggcagtgaaggaagaaactcagcagctagaggaagaactagaggccct
ggaagaaaggctggaaaaaattcaaaaggtccagttaaattgcactaagg
tgaagagtaagcaaagtgagcccagcaagcactcagggttttctacctca
gacaacagcatagccaacactccccaggattacagtgggaatatgaaatc
atttccatcccggagcccttcacaaggcgatgaagattctgctctgattc
taacccaagacaatctgaaaagttcagatccagatctgtcagccaacagt
gaccaagagtccggggtggaggatttgagctgtcggtctccaagtggtgg
tgagcatgcaccgagtgaagatagtggcaaggaccgggattctgatgaag
ccgtgtttctcactgcctgaagtttccctttggagttccaaagtaaagga
cacataagcaacacttccaaaaacaagggaacaaggtggtttattgtaaa
aacaggaaatggtgcatgtcattgagaactattttaatgcagctatgaaa
agggaaaaaagtgcccagttcttgatttcttagatactgaagaggacgta
gtcatttcatttatcaaatataaggaaaattattcaccattttgaagctc
accctagactatgaaaattatattcactgcagagcaattacttctgtcat
tacctgaagtgatcagtatctatcttccttgtcatagcatgcatctctca
aaaagcctccactcctttccctcacatctgtgatcatcatgattctttta
gttcacttctagatgcatattttgtgttttctaaagcatctgacattatc
ctcctttccgaccctcttatacatatttctaaaaacaggcacattggtga
gatgcaccctttttagttaatagatgcattcctaaggagcttttaattgc
ttatctttcaggcataatcatcactttaacttttccttggagcatatatt
ttgaattgtgagaataattttgttgcttttctctgagatctatagtctgt
ttctcctcattatttaaaaatgctaaaccttgtatctcactttttctcta
acactgatttaatagctaacgaggtagaagcaacattcattctcctggtc
ttacatatgaatttaagtatcagctttcttgtaataaccttttattactg
ttctagagactacactaccgacagtgtgggccagccaccagcctgatctc
aaagtatcacattataaagttagtagataaaacatctgtgagtgaaaatc
cagtttcaggaaccagagaattgggttgtcatgtctgtttaatgaaggga
ataggttttgtaatctatcattttagaaattatgtaactggctaatatgg
tttaattaaccttagtaacatctcgtaatctatcattttagaaattatgtaactggctaatatgg
tttaattaaccttagtaacatctcgtgaccactgactgctgaaagttctg
aaaagaatttttgttttgttacactgcacatttaagggagagtccctccc
ctatcttatgagttaaaaaagacttcactaggtgacctaaattaaactta
gtggggaaaagtggccatgtttggacataaataaatggtattcacactgt
atggttttaatatattagtacattctagaatgtaaaaggattaaacttta
caatttagatcaatattttgaatatgtgaaaggattaatttaaactttac
aatttacatcaatattttgaatatctgattttttttaatgggagaattat
tacatttcgctgaaatgaggacgagggcaagaaagcaacattgctgatct
ctctagtatgaaagatttggagggagtgttgcaatatatataaatgaaaa
catttaattgtgttcatcatatttaaaaatatagaatatattagagaact
gtgatttaaaagtactgttaatgtaaaaaataaagcaagtgtaattaatt
ctttcagaatataaaatttgggcattctctgctgagcagttcccaaatta
agtacaaggaatgtttattcattttctgcaatatactatatgtaataggg
aataccttgctaaaataaaacttaggatatagtggtaatggctttcacat
ttttataacataacataactcacttcacaaccttcttggagctgtccact
cttagaaactctgttgcctaatattgaggatgtggctttaatttcttccg
tttgacagtgtatgtctataaaaacaataaacattttttaaaaaatgaca
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa

 

Protein Sequence (660 aa):

MEHIRTPKVENVRLVDRVSPKKAALGTLYLTATHVIFVENSPDA
RKETWILHSQISTIEKQATTATGCPLLIRCKNFQIIQLIIPQERDCHDVYISLIRLAR
PVKYEELYCFSFNPMLDKEEREQGWVLIDLSEEYTRMGLPNHYWQLSDVNRDYRVCDS
YPTELYVPKSATAHIIVGSSKFRSRRRFPVLSYYYKDNHASICRSSQPLSGFSARCLE
DEQMLQAIRKANPGSDFVYVVDARPKLNAMANRAAGKGYENEDNYSNIKFQFIGIENI
HVMRNSLQKMLEVCELKSPSMSDFLWGLENSGWLRHIKAIMDAGIFIAKAVSEEGASV
LVHCSDGWDRTAQVCSVASLLLDPHYRTLKGFMVLIEKDWISFGHKFNHRYGNLDGDP
KEISPVIDQFIECVWQLMEQFPCAFEFNERFLIHIQHHIYSCQFGNFLCNSQKERREL
KIQERTYSLWAHLWKNRADYLNPLFRADHSQTQGTLHLPTTPCNFMYKFWSGMYNRFE
KGMQPRQSVTDYLMAVKEETQQLEEELEALEERLEKIQKVQLNCTKVKSKQSEPSKHS
GFSTSDNSIANTPQDYSGNMKSFPSRSPSQGDEDSALILTQDNLKSSDPDLSANSDQE
SGVEDLSCRSPSGGEHAPSEDSGKDRDSDEAVFLTA

 

SUBCLONING STRATEGY FOR YEAST TWO-HYBRID SCREENING AND CO-IP/CO-IF

LOCALIZATION IN HELA CELLS

SUBCLONED CONSTRUCTS

 

 

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