PCR from Human Liver
cDNA sequence:
gctatgccgctgcagggaaaatgctgagccctcccgggccgggtgggcgg
cggccggcgagggcggcgacggggacccgcttcccgagcgcggcggcggc
ggccatggcccggctggctgactacttcatcgtggtaggctatgaccacg
agaagccaggatcaggagaaggtctggggaaaataatccagagatttcca
cagaaggactgggatgatacaccttttccacagggaattgagttgttttg
tcagcctggcgggtggcagctgtccagagagaggaagcagccaacgttct
ttgtggttgtcctgacagacattgactcagatcgacattactgctcatgc
ctaaccttctatgaggcagagatcaatcttcagggaacaaagaaggaaga
gattgaaggtgaagcaaaagtgtctggtttaattcagcctgcagaagtgt
ttgctcccaaaagcctggtgttggtatccagattatattatccagaaatt
tttagggcttgcctgggtttgatctataccgtgtatgtggacagcctgaa
tgtctccttggaaagtctaattgcaaacctttgtgcctgccttgtcccag
cggctggagggtctcagaagctgttttctttgggtgcaggagatagacag
ttgatccagactcctttacatgatagtcttcctatcacgggcactagtgt
ggctctcctgttccagcaattgggaattcaaaatgtcctcagcctctttt
gtgcagtcctcacagaaaataaggttctcttccattctgcaagtttccag
agacttagtgatgcttgtagagccctggaatctttaatgtttcctcttaa
atatagttatccttatatccctattctcccggctcagctactggaagttc
taagttccccaacgcctttcattattggagtacattctgtctttaaaact
gatgtccatgaacttttagatgtaatcatagcagatttggatggaggcac
tattaaaattcccgaatgtattcacctctcttccctcccagaaccacttc
tacatcagactcaatcagctctttctttgattttacacccagatttggaa
gtagcagatcatgcttttcctcctccacgaacagctttatcccactcaaa
aatgctggataaagaggtgcgagccgttttccttagattatttgcacaac
tcttccaaggatatagatcctgcctgcaacttataagaattcatgcagag
ccagtaatacatttccacaagacagcattcttggggcagcgtggtttggt
cgagaatgatttcctcactaaagtactcagtggaatggcatttgcaggtt
ttgtttcagaaagaggtcctccttatagatcttgtgatctctttgatgag
ttggtagcctttgaagtagagagaattaaagttgaagaaaataacccagt
gaagatgataaagcatgtcagggaacttgctgagcaactattcaaaaatg
agaatccaaatcctcatatggcattccagaaagttccacggccaacagaa
ggatcccatttgcgagttcatattcttcctttcccagagattaatgaagc
ccgggttcaggaattaatacaggaaaatgttgctaagaaccagaatgcac
ctcctgccacacgaatagaaaagaaatgtgttgtgccagcaggtccacct
gttgtttcgataatggacaaggtgacgacagttttcaacagtgcacaaag
actagaagttgtcagaaactgtatctcattcatatttgaaaataaaattt
tggaaactgaaaagacccttcctgctgcactcagagcccttaaaggaaag
gcagcaagacagtgtctcactgatgaattgggtttgcatgtccagcaaaa
ccgggcaatattagaccatcaacagtttgactacataataaggatgatga
attgtactctacaggattgttcaagtttagaagaatacaacattgccgca
gcattactccctttgaccagtgctttctataggaaacttgcccctggagt
cagccagtttgcttacacgtgtgtacaagaccaccccatttggacaaatc
agcaattttgggagacaaccttttacaatgcagtgcaggaacaggttcgc
tccctttatctctcagccaaggaagacaatcatgccccgcatctgaagca
aaaggataagcttcctgatgaccattatcaggagaagacagcaatggacc
tggcagctgagcaactacgcctttggcctaccctgagcaagtcaactcag
caagagctagtgcaacatgaggaaagcactgtctttagtcaggccattca
ctttgcaaacctcatggtgaacctgctagttccactcgacacaagtaaaa
acaagctcctaagaacatcagcgccaggtgactgggagagcggaagcaac
agcattgtcacaaacagtattgcaggaagtgtagctgagagctatgatac
agagagtgggtttgaagattcagagaatactgacattgccaattctgttg
tgcggttcattacccgatttattgacaaagtttgtacagagagtggagtt
actcaggatcacatcaagagccttcattgcatgataccaggaattgtagc
tatgcacattgagaccctagaagcagtacatcgagaaagcagaagacttc
cgcctattcagaagcccaagattcttagacctgctctgctgccaggagaa
gaaattgtctgtgagggtcttcgagtcttgctggatcctgatggaagaga
agaagctactggaggtcttcttggaggccctcagctcctgccagcagaag
gagccttgttcctcaccacatacagaattctcttcagaggaacaccccat
gatcagttagtgggtgagcagacagttgtgcggagctttcccattgcctc
catcaccaaggagaagaagattacaatgcagaaccagctacagcagaaca
tgcaagaaggactgcagatcacatcagcatcttttcagttgattaaggta
gcatttgatgaagaagtcagtccagaagtagtagagatctttaagaaaca
gctgatgaagttccgttatcctcagtccattttcagtacctttgcttttg
ctgctggacaaactaccccacaaataattttaccaaaacagaaggaaaag
aacacttcttttcgtaccttctcaaaaacaattgtgaaaggtgccaaaag
ggcagggaaaatgacaattgggcggcaatatttactgaagaagaagacag
ggacaattgtggaagaaagagtaaatcgtcctggatggaatgaagatgat
gatgtatctgtttcagatgagagtgagctccccacaagtaccaccctgaa
ggcctccgagaagtctacaatggaacagttggtggaaaaagcttgtttca
gagactatcagcgtttaggtttaggaaccataagtggcagctcttcccgt
tcaagacccgagtattttagaattactgcctccaacaggatgtattcact
ctgccggagctatcctggccttttagtcgtacctcaagctgtacaggaca
gtagtttaccaagagtagctcgctgctatcgacacaatcgcctgcctgtt
gtatgttggaagaactcaagaagtggtactctgctcctccgatctggagg
attccatgggaagggagtcgttggtcttttcaaatctcagaactcccctc
aggccgctcctacctcctctttagaatcttccagtagcatagaacaagag
aaatacttgcaagccttactgaatgctgtttctgtccatcagaaactcag
aggcaacagcactcttactgtcaggccagcctttgctctatctccaggtg
tgtgggcaagtcttcgctctagcactcgcttgatcagctctccaacatcc
ttcattgatgttggcgcccggctggcaggcaaggatcactcggcctcctt
cagtaacagcagctacctacaaaaccagctcttgaaacggcaagcagccc
tttacatatttggtgaaaagtcgcaactaaggaacttcaaggtagaattt
gctttaaattgtgagtttgttcctgttgaatttcatgaaatccggcaagt
gaaagccagttttaagaagctgatgagggcttgtatcccaagcaccatcc
ctactgactcagaagtgaccttcctgaaagcgctgggagattctgagtgg
ttcccacagcttcacaggataatgcagctggctgtggttgtatcagaagt
acttgagaatggttcctcagttttggtctgtttggaggaaggctgggaca
tcactgcacaagtgacatccctggttcagttactcagtgatcccttttat
aggacacttgaaggcttccagatgttggttgaaaaagagtggctctcttt
tggtcacaaattcagtcagaggagcagcttgaccctcaactgtcagggga
gtggttttgctccagtcttcttacagttcttagactgtgtacaccaggtt
cacaaccagtatccaactgagtttgaattcaatctctattacttaaagtt
cttggctttccactatgtgtctaatcgctttaaaacatttctcctggatt
cagactatgaaagattagagcacggaactttatttgatgataaaggagaa
aagcatgccaaaaaaggagtctgtatttgggaatgtattgacagaatgca
caagaggagtcccattttctttaattatttatattcaccattggaaatag
aggctctaaagcccaatgtaaacgtctctagcctcaagaagtgggattac
tacatagaagagaccctgtccacaggcccttcctatgactggatgatgct
aacccccaagcacttcccctccgaagactctgacctggctggagaagctg
ggccacggagccagaggagaacagtgtggccatgctatgatgatgtcagc
tgtactcagcctgatgctctcaccagccttttcagtgaaattgaaaaatt
ggagcacaaattgaaccaagcccctgagaagtggcagcagctgtgggaaa
gggtaaccgtggaccttaaagaagaaccaagaacagatcgctcccaaaga
cacctgtcgagatccccaggaattgtgtctaccaacctaccttcctatca
gaagaggtctctgctacatctcccagacagcagcatgggggaggaacaga
attccagcatctccccatccaatggagtggagcgaagagcagccacgctc
tatagccagtatacatccaagaatgatgaaaacaggtcctttgagggaac
actttataaaagaggggctttgctgaaaggttggaagccccgttggtttg
ttttggatgtaacaaaacatcagctgcgctactatgactcaggtgaggac
acaagctgtaaaggccacattgatctggctgaagtagaaatggtcatccc
tgctggccccagcatgggagccccaaagcacacaagtgacaaggctttct
ttgatctcaagaccagcaaacgtgtgtataacttctgcgcccaggatgga
cagagtgcccagcaatggatggacaagatccagagttgtatctctgatgc
ctgatgcccatggtcaacccacgcagaagaaacagaagaactcatgctgc
cagatagatagaaaaagaagcatggatccttgaggagctgacaacaagtt
atcccagggcctgaggttctcctgcccagtcccctcttgcaggggttgct
atatctacttaacctgaataggtgtttcacacaggtctggtcaacagccc
catgcactccctgtatcttgcactaaatttttctaacagggtcttagtgg
ttaatgatcagaagatgtctcctgagccaactgtgaacctcacccaggca
aaatggctaccacctacttgggtccttcttcatgaaagctatagatcctt
ttttgttctctgaggtcataatttcctcggagacctgtttaacaagcaaa
aatcaaaaccctccaagattgtctcatattctacctggactaggtttcct
atgagagacatctacttgtaatgcctgacctttgagatgctcagttctct
ggtgctgccaaaagatgcttccatggtccgtgctctgccagtggggttca
caacaagagacgtcattgttcagtagcaggcaaagagggagcacacagca
ttattctgatggaaaaagattatccagggaatggtacaacaatgaccagc
ccaatgcaggaaaacactacttccaaaacactgaattctctagaccagag
gtgctctgaggatccagggccttgtgttcttatgtatcttctgcttcctg
acagcttctttttcaaaataacatgcaaaaaaagctgaatgcactaactc
acaaaacaaacacttgcactgaattcccaatgaagtgaagatgttggaaa
gacagaggccagctatttaggaccatacgcacctgtgacaagggctgtgt
tgaccacagtcacactgtggcatgactggatacccaaactacacttctac
acatgaaaagtaagaactgtctttagattttctttactttgataacttgt
gattgtttagcttaagacccaagaaatgctgtttgctcatggtaaacaga
aacagcatcttcgctacaaccactgacaccagctggcgtcataggtagct
agatcattgcatttgttttgaaatgttaatatgttaaatactaaactaat
atttcaaaaatgtgtatatatgatttctatatccttgtttttcagatagc
ctgcttataatttaatataaattaactgatgcattcataagatttcaata
atgaaatggttcccttttaaaaaataaaaatactttgtagattaaaaata
aatcagaatttcaaatttaaaattgtccacacactaggaaatagaactgt
gttaatatataagaaatctggggataattaagaatgaaggacttttctat
catttccattttataaattgccacctgtgaaaatggtttttgcacattat
ttgtatttttctttgtatatgaaataattttttgtactttgtaaaatatg
gagcccattgtaccttcagctatttgagactatacacagtgcttcttttg
taactggattactttaactttcgtgaaggcattacattgcctcacattca
ctaaccaccttgaattaaatttatttcttaagaaaaagcatccctcattt
actgtgaactaaagttcattctgtagtgcacagcattcaggagggtaaca
ctcaggattcatacatgggactaaaggttcaagaataaaacaaagcatga
cccac
Protein Sequence (1949 aa):
marladyfivvgydhekpgsgeglgkiiqrfpqkdwddtpfpqgielfcq
pggwqlsrerkqptffvvvltdidsdrhycscltfyeaeinlqgtkkeei
egeakvsgliqpaevfapkslvlvsrlyypeifraclgliytvyvdslnv
sleslianlcaclvpaaggsqklfslgagdrqliqtplhdslpitgtsva
llfqqlgiqnvlslfcavltenkvlfhsasfqrlsdacraleslmfplky
sypyipilpaqllevlssptpfiigvhsvfktdvhelldviiadldggti
kipecihlsslpepllhqtqsalslilhpdlevadhafppprtalshskm
ldkevravflrlfaqlfqgyrsclqlirihaepvihfhktaflgqrglve
ndfltkvlsgmafagfvsergppyrscdlfdelvafeverikveennpvk
mikhvrelaeqlfknenpnphmafqkvprptegshlrvhilpfpeinear
vqeliqenvaknqnappatriekkcvvpagppvvsimdkvttvfnsaqrl
evvrncisfifenkiletektlpaalralkgkaarqcltdelglhvqqnr
aildhqqfdyiirmmnctlqdcssleeyniaaallpltsafyrklapgvs
qfaytcvqdhpiwtnqqfwettfynavqeqvrslylsakednhaphlkqk
dklpddhyqektamdlaaeqlrlwptlskstqqelvqheestvfsqaihf
anlmvnllvpldtsknkllrtsapgdwesgsnsivtnsiagsvaesydte
sgfedsentdiansvvrfitrfidkvctesgvtqdhikslhcmipgivam
hietleavhresrrlppiqkpkilrpallpgeeivceglrvlldpdgree
atggllggpqllpaegalflttyrilfrgtphdqlvgeqtvvrsfpiasi
tkekkitmqnqlqqnmqeglqitsasfqlikvafdeevspevveifkkql
mkfrypqsifstfafaagqttpqiilpkqkekntsfrtfsktivkgakra
gkmtigrqyllkkktgtiveervnrpgwnedddvsvsdeselptsttlka
sekstmeqlvekacfrdyqrlglgtisgsssrsrpeyfritasnrmyslc
rsypgllvvpqavqdsslprvarcyrhnrlpvvcwknsrsgtlllrsggf
hgkgvvglfksqnspqaaptsslessssieqekylqallnavsvhqklrg
nstltvrpafalspgvwaslrsstrlissptsfidvgarlagkdhsasfs
nssylqnqllkrqaalyifgeksqlrnfkvefalncefvpvefheirqvk
asfkklmracipstiptdsevtflkalgdsewfpqlhrimqlavvvsevl
engssvlvcleegwditaqvtslvqllsdpfyrtlegfqmlvekewlsfg
hkfsqrssltlncqgsgfapvflqfldcvhqvhnqyptefefnlyylkfl
afhyvsnrfktflldsdyerlehgtlfddkgekhakkgvciwecidrmhk
rspiffnylyspleiealkpnvnvsslkkwdyyieetlstgpsydwmmlt
pkhfpsedsdlageagprsqrrtvwpcyddvsctqpdaltslfseiekle
hklnqapekwqqlwervtvdlkeeprtdrsqrhlsrspgivstnlpsyqk
rsllhlpdssmgeeqnssispsngverraatlysqytskndenrsfegtl
ykrgallkgwkprwfvldvtkhqlryydsgedtsckghidlaevemvipa
gpsmgapkhtsdkaffdlktskrvynfcaqdgqsaqqwmdkiqscisda
SUBCLONING STRATEGY FOR YEAST TWO-HYBRID SCREENING AND CO-IP/CO-IF