Clostridium difficile is a Gram-positive spore forming bacterium and an aetiological cause of antibiotic-associated pseudomembranous colitis in humans. Its pathogenicity is attributed to the production of exotoxins, which have a cytotoxic effect on mammalian cells. Owing to the emergence of hypervirulent strains with increased resistance to antibiotics, new control strategies are needed. The aim of this study was to develop a C. difficile control strategy involving the delivery of C. difficile endolysin to the gastro-intestinal tract. The gene encoding the lysin together with a signal peptide of Lactobacillus origin was cloned and expressed in Lactococcus Lactis. Lysin secretion was detected by an endolysin plate assay, based on the seeding of heat inactivated C. difficile cells in solid agar medium. A zone of inhibition around the recombinant L. lactis clones demonstrated the secretion of an active C. difficile endolysin to its surrounding environment. The effective secretion of C. difficile endolysin suggests that it may be developed as a potential tool for the control of C. difficile related illness in the gastro-intestinal tract.