The use of bacteriophages for decontamination of different surfaces
Introduction: The development of an alternative to antibiotics anti-microbial remedies has become one of the highest priorities of modern medicine and biotechnology. One of such alternatives might be bacteriophages. Phage application was reported highly efficient, highly specific, and cost-effective when applied for pathogens eradication in food products and water, in medicine, and in veterinary. Recently, volumetric decontamination of confined environment, such as hospitals, transportation units, and public buildings raised substantial interest because it allows to simultaneously decontaminating pathogens, including in hardly accessible sites.
Aim: Our aim was exploration of bacteriophage-containing microaerosol for reduction of high load of Salmonella cells on inanimate surfaces. We limitedly explored volumetric disinfection of S. typhimurium cells inoculated on hard surfaces and on the surfaces of rich organic medium, using microaerosol containing Salmonella phage.
Materials and Methods: Fresh S. typhimurium cells were inoculated on a surface of nutrition medium and also, were inoculated and predried on microscopic glass. All coupons were placed to enclosed plastic chamber and microaerosol composed of Salmonella bacteriophage Sal.phi18 was fumigated to the chamber using nebulizer. Multiple controls were applied, such as cells survival on the coupons that had not been exposed to bacteriophage, fumigation of microaerosol not-containing phage, etc.
Results and Summary: The results of the trial illustrated above represent unambiguous evidence of the efficacy of the microaerosol composed of bacteriophage to reduce high load of Salmonella cells on glass surface and on rich nutrition medium below detection limit. Predictably, exposure of the coupons to microaerosol containing no phages had neither positive, nor negative effect to viability of the cells inoculated on glass surface and on nutrition medium. Successful results of such microaerosol application presumably will expand bacteriophage application as environmentally safe disinfectants. Use of phages for volumetric disinfection might also find an application for preventing airborne pathogens dissemination.
Aim: Our aim was exploration of bacteriophage-containing microaerosol for reduction of high load of Salmonella cells on inanimate surfaces. We limitedly explored volumetric disinfection of S. typhimurium cells inoculated on hard surfaces and on the surfaces of rich organic medium, using microaerosol containing Salmonella phage.
Materials and Methods: Fresh S. typhimurium cells were inoculated on a surface of nutrition medium and also, were inoculated and predried on microscopic glass. All coupons were placed to enclosed plastic chamber and microaerosol composed of Salmonella bacteriophage Sal.phi18 was fumigated to the chamber using nebulizer. Multiple controls were applied, such as cells survival on the coupons that had not been exposed to bacteriophage, fumigation of microaerosol not-containing phage, etc.
Results and Summary: The results of the trial illustrated above represent unambiguous evidence of the efficacy of the microaerosol composed of bacteriophage to reduce high load of Salmonella cells on glass surface and on rich nutrition medium below detection limit. Predictably, exposure of the coupons to microaerosol containing no phages had neither positive, nor negative effect to viability of the cells inoculated on glass surface and on nutrition medium. Successful results of such microaerosol application presumably will expand bacteriophage application as environmentally safe disinfectants. Use of phages for volumetric disinfection might also find an application for preventing airborne pathogens dissemination.
Reference:
Poster Day 4-T12-Pos-23
Session:
Posters Covering the use of viruses to control infection and Processes governing the applied use of viruses
Presenters:
Taras Gabisonia
Session:
Day 4 Posters Covering: The use of viruses to control infection and Processes governing the applied use of viruses
Presentation type:
Poster presentation
Room:
Poster Halls
Date:
Thursday, 21 July 2016
Time:
12:05 - 15:30