Recently, it has report for the first time plasmid-mediated colistin resistance encoded by the mcr-1 gene in Escherichia coli isolates from humans, animals and foodstuffs in China. Here, we use CRISPR-Cas technology to create antimicrobials whose spectrum of activity is chosen by design. The DNA targets of gRNAs can be undesirable genes or polymorphisms, including antibiotic resistance and virulence determinants in Escherichia coli. Truncated guided RNAs (gRNAs) targeting specific DNA sequences of mcr-1 gene are delivered efficiently to microbial populations using Nisin which producted by Lactococcus lactis.