Food-borne pathogen having shiga toxin (stx) has caused diarrhea and hemolytic uremic syndrome. E. coli O157:H7 was the main cause of these diseases, but the outbreaks by shiga toxin-producing non-O157 E. coli (STEC) have also been reported recently. Stx genes from bacteriophages might suggested to be transferred to non-pathogenic host bacteria. To understand the safety of bacteriophage in the environments, bacteriophage were isolated, transferred and expressed in E. coli. 22 bacteriophages for E. coli host infection were isolated from river waters and waste waters. Stx genes encoded in the bacteriophages were transferred to eight non-pathogenic host bacteria, which were non-O157 E. coli and did not be encoded with shiga toxin gene. 11 transductants were detected. Transferred non-O157 E. coli by NOECP49 phage was encoded with stx1 and stx2 genes. When the E. coli was exposed in osmotic and heat shock conditions, stx1 and stx2 toxin were expressed. The E. coli was incubated at salt conditions of 1%, 3%, 5%, and 7% during 16 hrs. Stx1 toxin expressed average 40 titer at salt conditions of 1%~7%. Stx2 toxin was expressed average 34.7 titer at salt condition of 1%~5%, but it wasn’t detected at 7% salt. Also the E. coli was treated at heat shock conditions of 40℃, 50℃, and 60℃. At higher temperature, more stx1 and stx2 toxins were detected. As a result, shiga toxin genes of non-O157 E. coli transferred from phage were expressed in such the conditions. Therefore, bacteriophage in the environment might be transferred to E. coli and be related to the food-borne diseases.