The resistance to antibiotics in clinically important bacteria is one of the major global health concerns. Phage therapy could be one such reliable alternative therapeutic strategy to combating the superbugs. In the following study, we assessed the host range of a novel bacteriophage, JHP, and its characterization was performed to be used in phage therapy. The bacteriophage showed infectivity over a broad range of genera including multidrug resistant clinical isolates of Pseudomonas aeruginosa, members of family Enterobacteracae and other important human pathogens. The antibacterial activity was highest at pH 7, and temperature of 37°C. The phage lytic activity gradually decreased till 60°C and showed no activity when temperature further raised. The bacteriophage could safely be stored a 4 or -20°C. The latent period of the bacteriophages was 25 minutes and showed a burst size 433 virions per cell. The size of JHP genome was approximately 30kb in length. Evidenced by the electron microscope image, the phage has an icosahedral head with non-contractile tail, which put it in family, Siphoviridae. The diameter of JHP head was found to be 115nm and its tail length 152nm. To sum up, the broad spectrum Siphoviridae phage JHP is ingenious candidate for phage therapy.