The clinical success of phage therapy is dependent on the development of strategies able to overcome the limitations of (bacterio)phages as therapeutic agents. Many approaches have been proposed to circumvent phages’ intrinsic limitations but none have proved to be completely satisfactory. To achieve that, 15 diverse phages were isolated from natural sources against the standard Escherichia coli reference collection (ECOR), all of which were restricted to infecting E. coli strains. Among the phages evaluated against 75 Enterobacteriaceae related strains, three complementary promising virulent broad range phages were selected for genome scrambling - vB_WUR_E36s, vB_WUR_E55l and vB_WUR_E55s. Random recombination of DNA fragments of these phages allowed the assembly of new viral particles. These were characterized and insights into phage-host interactions, evolution and genome organization are discussed. We further demonstrate the application of our method with natural isolated unknown viral fractions. To conclude, we developed a method to create chimeric phages not only to circumvent the major hurdles of phage limited specificity but also as an achievable strategy to shape phages to exhibit desirable biological properties.