U.K. Centre for Tissue Engineering (UKCTE )

The Institute of Ageing and Chronic Disease

University of Liverpool
Duncan Building
Daulby Street
LIVERPOOL L69 3GA
United Kingdom

 

@JohnHuntUKCTE

Email: huntja@liv.ac.uk

www.liv.ac.uk/research/research-themes/materials-for-the-future/


Professor John Hunt D.Sc. FBSC
Head of Unit


imageanalysis01microscope1

Research Interests

One important area of biomaterials research is assessing the performance of materials with respect to their potential to affect the cells and tissue with which they are intended to come into contact. This requires a complex and flexible series of analytical techniques, that can accommodate the different forms a material may be presented in, and the different sites within a body a material will be placed: a useful material for use as a vascular graft may not be expected to make a good intraoccular lens.

Microscopically, the interactions of materials with bodily tissues are investigated by a combination of immunohistochemistry, in situ hybridisation, flow cytometry, confocal microscopy and image analysis. Characteristically, the tissue response to an implanted material is one of inflammation, with some final equilibration between the material and the tissue, and this is not necessarily ideal for the tissue or material. The response is characterised non-subjectively by using a panel of monoclonal antibodies to stain for specific cell types that are potentially involved in the host's response.

oz21w4ed2

Following identification, the cellular response is quantified using computer image processing and analysis that can select and count the stained cells and return their position with respect to the implanted material. This is a particularly involved technique that requires unique routines, written to select and count each cell type observed in the tissue response.

From this analysis, the type and magnitude of the response is determined and compared to control materials. The analysis also returns information on the position of these cells with respect to the implanted material, which are then mathematically modelled to determine how the material relates to other materials with the same intended purpose/function. Flow cytometry can be utilised to look in more detail at receptor expression on these inflammatory cells and the real size and granularity of these cells can also be determined.

 

Research Links

Research Opportunities (Currently available Research Studentships)

Internet Media Links

Journal Publications

·         Bryan N, Ashwin H, Smart N, Bayon Y, Wohlert S, Hunt JA. The in vivo evaluation of tissue-based biomaterials in a rat full-thickness abdominal wall defect model. J Biomed Mater Res B Appl Biomater. 2013 Oct 24. doi: 10.1002/jbm.b.33050

·         Bryan N, Ashwin H, Chen R, Smart NJ, Bayon Y, Wohlert S, Hunt JA. Evaluation of six synthetic surgical meshes implanted subcutaneously in a rat model.J Tissue Eng Regen Med. 2013 Oct 7. doi: 10.1002/term.1807

·         Curran JM, Fawcett S, Hamilton L, Rhodes NP, Rahman CV, Alexander M, Shakesheff K, Hunt JA. The osteogenic response of mesenchymal stem cells to an injectable PLGA bone regeneration system. Biomaterials. 2013 Dec;34(37):9352-64. doi: 10.1016/j.biomaterials.2013.08.044.

·         Bryan N, Lewis FC, Bond D, Stanley C, Hunt JA. Evaluation of a novel non-destructive catch and release technology for harvesting autologous adult stem cells. PLoS One. 2013, 8(1):e53933. doi: 10.1371/journal.pone.0053933. Epub 2013 Jan 22

·         Cachinho SC, Pu F, Hunt JA. Cytokine secretion from human peripheral blood mononuclear cells cultured in vitro with metal particles. J Biomed Mater Res A. 2013 Apr;101(4):1201-9. doi: 10.1002/jbm.a.34410. Epub 2013 Jan 24.

·         Bryan N, Ashwin H, Smart N, Bayon Y, Scarborough N, Hunt JA. The innate oxygen dependant immune pathway as a sensitive parameter to predict the performance of biological graft materials. Biomaterials. 2012 Sep;33(27):6380-92. doi: 10.1016/j.biomaterials.2012.05.058. Epub 2012 Jun 26.

·         Lewis F.C. Bryan N. Hunt J.A. A Feeder-Free, Human Plasma-Derived Hydrogel for Maintenance of a Human Embryonic Stem Cell Phenotype In Vitro. Cell Regeneration 2012, 1:6.  doi:10.1186/2045-9769-1-6. 

·         Chen R. Bayon Y. Hunt J.A.  Preliminary study on the effects of ageing cold oxygen plasma treated PET/PP with respect to protein adsorption. Colloids Surf B Biointerfaces. 2012 Aug 1;96:62-8. Epub 2012 Apr 6.

·         Bryan N. Birch P. Stanley C. Bond D. Hunt J.A. The use of acoustic force capture to ultra-purify lymphocyte subpopulations from human adult whole blood. J Tissue Eng Regen Med. 2012 Mar 21. doi: 10.1002/term.1474.

·         Bryan N. Ahswin H. Smart N.J. Bayon Y. Hunt J.A. In vitro activation of human leukocytes in response to contact with synthetic hernia meshes. Clin Biochem. 2012 Jun;45(9):672-6. Epub 2012 Mar 9.

·         Navarro M. Pu. F. Hunt J.A. The Significance of the Host Inflammatory Response on the Therapeutic Efficacy of Cell Therapies Utilising Human Adult Stem Cells. Experimental Cell Research. 2012 318(4) 361-70. http://dx.doi.org/10.1016/j.yexcr.2011.12.006 

·         Bryan N, Ashwin H, Smart N, Bayon Y, Wohlert S, Hunt JA. Reactive oxygen species (ROS)--a family of fate deciding molecules pivotal in constructive inflammation and wound healing. Eur Cell Mater. 2012 Sep 24;24:249-65.

·         Wilson P.J.M. Jarvis J.C. Davidson J.S. Hunt J.A. Ranganath L.R. & Gallagher J.A. The Role of Calcified Cartilage and Subchondral Bone in the Initiation and Progression of Ochronotic Arthropathy in Alkaptonuria  Arthritis and Rheumatism 2011 63(12) 3887-3896.  doi: 10.1002/art.30606.

·         Curran J.M. Pu F. Chen R. Hunt J.A. The use of dynamic surface chemistries to control MSC isolation and function. Biomaterials. 2011 Jul; 32(21):4753-60.   http://dx.doi.org/10.1016/j.biomaterials.2011.03.045

·         Rhodes N.P. Hunt J.A. Longinotti C. Pavesio A. In Vivo Characterization of Hyalonect, a Novel Biodegradable Surgical Mesh. Journal of Surgical Research, J Surg Res. 2011 Jun 1;168(1):e31-8. Epub 2010 Oct 8.

·         Halfhide C.P. Flanagan B.F. Brearey S.P. Hunt J.A. Fonceca A.M. McNamara P.S. Howarth D. Edwards S. Smyth R.L. Respiratory Syncytial Virus binds and undergoes transcription in neutrophils in the blood and airways of infants with severe bronchiolitis. Journal of Infectious Diseases 2011 204 451-458.

·         Bryan N, Andrews K, Loughran MJ, Rhodes NP, Hunt JA Elucidating the Contribution of the Elemental Composition of Fetal Calf Serum Towards Antigenic Expression of Primary Human Umbilical Vein Endothelial Cells In Vitro. Biosci Rep. 2011 31(3) 199-210.

·         Curran J.M. Stokes R. Irvine E. Graham D. Amro N.A. Sanedrin R.G. Jamil H. and Hunt J.A. Introducing dip pen nanolithography as a tool for controlling stem cell behaviour: unlocking the potential of the next generation of smart materials in regenerative medicine Lab on a Chip 2010 10 1662-1670 doi:10.1039/c004149a

·         Alves C.M. Reis R.L. Hunt J.A.  The dynamics, kinetics and reversibility of protein adsorption onto the surface of biodegradable materials Soft Matter 2010 6(17) 4135-4143.

·         Alves C. Reis R. Hunt J.A. The Competitive Adsorption of Human Proteins onto Natural-Based Biomaterials.  J. R. Soc. Interface 2010. 7(50) 1367-1377.

·         Pu F, Rhodes NP, Bayon Y, Chen R, Brans G, Benne R, Hunt JA The use of flow perfusion culture and subcutaneous implantation with fibroblast-seeded PLLA-collagen 3D scaffolds for abdominal wall repair Biomaterials. 2010 May;31(15):4330.

·         Rhodes N.P. Hunt J.A. Longinotti C. Pavesio A. In Vivo Characterization of Hyalonect, a Novel Biodegradable Surgical Mesh. Journal of Surgical Research, In Press, Uncorrected Proof, Available online 8 October 2010.

·         Curran J.M. Chen R. Hunt J.A. Material Induced Mesenchymal Stem Cell Differentiation. Biomaterials 2010 31 1463-1464 doi:10.1016/j.biomaterials.2009.12.001   

·         Donegan GC, Hunt JA, Rhodes N.P. Investigating the importance of flow when utilizing hyaluronan scaffolds for tissue engineering. Journal of Tissue Engineering and Regenerative Medicine 2010 4(2), 83 – 95.

·         Curran J.M. Chen R. Stokes R. Irvine E. Graham D. Gubbins E. Delaney D Amro N. Sanedrin R. Jamil H. Hunt J.A. Nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations. Journal of Materials Science: Materials in Medicine 2010 21(3) 1021. doi:10.1007/s10856-009-3976-x

·         O'Hare, P., Meenan, B.J., Burke, G.A., Byrne, G., Dowling, D., Hunt, J.A. Biological responses to Hydroxyapatite surfaces deposited via a co-incident microblasting technique. Biomaterials 2010, 31(3) 515-522. doi:10.1016/j.biomaterials.2009.09.067

·         Halfhide C. P. Brearey S.P. Flanagan B.F. Hunt J.A. Howarth D. Cummerson J. Edwards S. Hart C.A. Smyth R.L. Neutrophil TLR4 protein expression is reduced in the airways of infants with severe bronchiolitis. Thorax. 2009 Sep;64(9):798-805.

·         Curran, J.M. Tang, Z. Hunt, J.A. PLGA doping of PCL affects the plastic potential of human mesenchymal stem cells, both in the presence and absence of biological stimuli. Journal of Biomedical Materials Research Part A, 2009 89(1) 1 -12. Doi: 10.1002/jmb.a.31966

·         Bryan N, Rhodes N.P. Hunt J.A. Derivation and performance of an entirely autologous injectable hydrogel delivery system or cell-based therapies. Biomaterials 2009, Jan;30(2) 180-188. doi:10.1016/j.biomaterials.2008.09.003

·         Hunt J.A. Regenerative medicine: Materials in a cellular world. Nature Materials 2008, Aug;7(8), 617-8. doi:10.1038/nmat2242

·         Human Adipose Precursor Cells Seeded on Hyaluronic Acid-Based (HYAFF TM 11) Spongy Scaffolds: a Pilot Clinical Trial. Stillaert F. Hunt J.A. Rhodes N.P. Di Bartolo C. Tognana E. Monstrey S. Blondeel P. Biomaterials
2008, 29(29), October, 3953-3959
doi:10.1016/j.biomaterials.2008.06.005

·         Andrews K.D. Feugier P. Black, R.A. Hunt J.A. Vascular Prostheses: Performance Related to Cell-Shear Responses. Journal of Surgical Research, 2008, 149(1), September, 39-46. doi:10.1016/j.jss.2007.08.030

·         Hunt J.A. Callaghan J.T.Polymer-Hydroxyapatite Composite versus Polymer Interference Screws in Anterior Cruciate Ligament Reconstruction in a Large Animal Model. Knee Surgery, Sports Traumatology, Arthroscopy. 2008. 16, 655-660 doi:10.1007/s00167-008-0528-8

·         Baldwin L, Hunt JA. The in vivo cytokine release profile following implantation. Cytokine 2008, 41, 217-222. doi:10.1016/j.cyto.2007.11.015

·         Rhodes NP, di Bartolo C, Bellini D, Hunt JA. Induction of adipose tissue regeneration by chemically-modified hyaluronic acid. International Journal of Nano and Biomaterials 2008. 1, 250-262.

·         Andrews K.D. Hunt J.A. Upregulation of Matrix and Adhesion Molecules Induced by Controlled Topography. Materials in Medicine 2008, 19, 1601-1608. Doi: 10.1007/s10856-008-3377-6

·         Andrews K.D. Black, R.A. Hunt J.A. The Technology of Electrostatic Spinning for Tissue Engineering Scaffolds.  Polymer International 2008, 57, 203-210. doi: 10.1002/pi.2317

·         S.M. Richardson, N. Hughes, J.A. Hunt, A.J. Freemont, J.A. Hoyland. Human Mesenchymal Stem Cell Differentiation to NP-like Cells in Chitosan-glycerophosphate Hydrogels.  Biomaterials, 2008, 29, 85-93.  doi: 10.1016/j.biomaterials.2007.09.018

·         Dennis von Heimburg, Karsten Hemmrich, Nicholas P. Rhodes, John A. Hunt, Chiara Di Bartolo, Norbert Pallua, and Phillip Blondeel. Autologous in vivo adipose tissue engineering in hyaluronan-based gels in a pig model. Journal of Surgical Research 2008, 144, 82-88. doi:10.1016/j.jss.2007.03.017

·         Hunt J.A. The Criticality of Quantitative Analysis and Reproducibility for the Development of Interactive Regenerative Treatments. Biomaterials, 2007, 28, 5128-5130. doi: 10.1016/j.biomaterials.2007.07.016 

·         R. Chen J.A. Hunt. Biomimetic Materials Processing for Tissue Engineering Processes. Journal of Materials Chemistry, 2007, 17, 3974 – 3979. doi: 10.1039/b706765h

·         McGee, J. A. Hunt. Bone Regeneration in a Bi-Lateral Sinus Lift; a Comparative Pilot Case Study Between Irradiated Human Cancellous Bone Versus a Synthetic Analogue. European Journal for Dental Implantologists. 2007, 3, 54-57.

·         D.M. Warde. S.E. Herrick. J.A. Hunt. Autologous Fat Transplantation and Adipose Tissue in Tissue Engineering. International Journal of Adipose Tissue Vol 1(1): 42-48.  2007.

·         Rhodes, N.P. Di Bartolo, C. Hunt, J.A. Analysis of the Cellular Infiltration of Benzyl-Esterified Hyaluronan Sponges Implanted in Rats Biomacromolecules,2007,  8 (9), 2733 -2738; doi: 10.1021/bm070368p

·         Tang Z.G. Rhodes N.P. Hunt J.A. Control of the domain microstructures of PLGA and PCL binary systems: Importance of morphology in controlled drug release. Trans IChemE, Part A, Chemical Engineering Research and Design, 2007, Vol 85(A7): 1044–1050. doi:10.1205.cherd06217

·         Mirsadraee s. Wilcox H.E. Watterson K.G. Kearney J.N. Hunt J.A. Fisher J. Ingham E. Biocompatibility of acellular human pericardium. Journal of Surgical Research 2007, 143, 407-414. doi:10.1016/j.jss.2007.01.026 

·         M. Oates, R. Chen, M. Duncan, J.A. Hunt. The Angiogenic Potential of Three Dimensional Open Porous Synthetic Matrix Materials.  Biomaterials Vol 28: 3679-3686. 2007. doi:10.1016/j.biomaterials.2007.04.042

·         K.D. Andrews, J.A. Hunt, R.A. Black. An Investigation into the Effects of Sterilisation on the Physical Properties of Electrostatically Spun Scaffolds. Biomaterials Vol 28: 1014-1026. 2007. doi:10.1016/j.biomaterials.2006.10.014 

·         Marina Brama, Nicholas Rhodes, John Hunt, Andrea Ricci, Silvia Migliaccio, Carlo Della Rocca, Roberto Teghil,  Silvia Leccisotti, Attilio Lioi, Marta Scandurra, Giovanni De Maria, Daniela Ferro, Fanrong Pu, Laura Politi and Roberto Scandurra. Effect of titanium carbide coating on the osseointegration response in vitro and in vivo. Biomaterials Vol 28: 595-608. 2007. doi:10.1016/j.biomaterials.2006.08.018 

·         Baldwin L. Hunt J.A. The inflammatory response to NiCr, CoCr and Ti upon implantation in Rats. J. Biomed. Materials Res. Vol 79: 574-581. 2006. doi:10.1002/jbm.a.30856

·         Curran J.M. Chen R. Hunt J.A. The Guidance of Human  Mesenchymal Stem Cell Differentiation in vitro by Controlled Modifications to the Cell Substrate. Biomaterials, Vol 27,4783-4793. 2006. doi:10.1016/j.biomaterials.2006.05.001 

·         Richardson S.M. Walker R.V. Parker S. Rhodes N. Hunt J.A. Freemont A.J. Hoyland J.A. Intervertebral Disc Cell Mediated Mesenchymal Stem Cell Differentiation. Stem Cells Vol  24: 707-716  2006. doi: 10.1634/stemcells.2005-0205

·         R. Chen, S. J. Curran, J. M. Curran, J. A. Hunt. The use of Poly(L-Lactide) and RGD Modified Microspheres as Cell Carriers in a Flow Intermittency Bioreactor for Tissue Engineering Cartilage. Biomaterials, Vol 27, 4453-4460. 2006. doi:10.1016/j.biomaterials.2006.04.011 

·         Tang Z.G. Hunt J.A. The Effect of PLGA doping of polycaprolactone films on the control of osteoblast cell adhesion and proliferation in vitro.  Biomaterials, Vol 27, Issue 4409-4418. 2006. doi:10.1016/j.biomaterials.2006.04.009 

·         Richardson, S.M. Curran, J.M. Chen, R. Vaughan-Thomas, A. Hunt, J.A. Freemont, A.J. and Hoyland, J.A. The differentiation of bone marrow mesenchymal stem cells into chondrocyte-like cells on poly-l-lactic acid (PLLA) scaffolds. Biomaterials, Vol 27, Issue 4069-4078. 2006. doi:10.1016/j.biomaterials.2006.03.017 

·         K. Fujiyoshi and J.A. Hunt. The Effect of Particulate Material on the Regulation of Chemokine Receptor Expression in Leukocytes. Biomaterials Vol 27 3888-3896. 2006. doi:10.1016/j.biomaterials.2006.02.047

·         Rhodes, N.P. Bellón, J.M. Buján, J. Soldani, G. Hunt, J.A.  Inflammatory Response to a Novel Series of Siloxane-Crosslinked Polyurethane Elastomers Having Controlled Biodegradation. Journal of Materials Science: Materials in Vol 16(12) 1207 – 1211. 2005. doi: 10.1007/s10856-005-4730-7

·         Marques, A.P. Reis, R.L. Hunt, J.A. The effect of starch-based biomaterials on leukocyte adhesion and activation in vitro. Journal of Materials Science: Materials in Vol 16(11) 1029 – 1043. 2005. doi: 10.1007/s10856-005-4757-9

·         Curran, S.J. Chen, R. Curran, J.M. Hunt, J.A. Expansion of Human Chondrocytes in an Intermittent Stirred Flow Bioreactor Using Modified Biodegradable Microspheres. Tissue Eng. September / October;11(9-10):1312-1322. 2005. doi: 10.1089/ten.2005.11.1312

·         Tang Z.G Callaghan J.T. Hunt J.A. The Physical Properties and Response of Osteoblasts to Solution Cast Films of PLGA Doped Polycaprolactone. Biomaterials Vol 26 6618-6624. 2005. doi:10.1016/j.biomaterials.2005.04.013 

·         Marques, A. P. Reis, R. L. Hunt, J. A. In Vivo study of the Host Response to Starch-Based Polymers and Composites Subcutaneously Implanted in Rats. Macromolecular Bioscience. Volume 5(8) 775 - 785. 2005. doi: 10.1002/mabi.200500010

·         Curran, J.M. Chen, R. Hunt, J.A. Controlling the phenotype and function of mesenchymal stem cells in vitro by adhesion to silane modified clean glass surfaces. Biomaterials Vol 26 7057-7067. 2005. doi:10.1016/j.biomaterials.2005.05.008

·         Hunt, J.A. Callaghan, J.T. Sutcliffe, C.S. Morgan, R.H. Halford, B. Black, R.A. Osseointegration of CoCr alloy implants with 3D surface topography defined by CAD-CAM. Biomaterials Vol 26, 5890-5897. 2005. doi:10.1016/j.biomaterials.2005.03.004 

·         Al-Badairy, H. Tatlock, G. Fawcett, S. Beahan, P. Hunt, J. FEG-SEM Investigation of α-alumina Scales Formed on FeCrAlY Aloys Oxidised at 12000 C.  J. Phys. IV France. Vol 124 17-24. 2005.

·         Baldwin, L. Flanagan, B.F. Hunt, J.A. Flow Cytometric Measurement of Phagocytosis Reveals a Role for C3b in Metal Particle Uptake by Phagocytes. J. Biomed. Materials Res. Vol 73(1) 80-85. 2005. doi: 10.1002/jbm.a.30252

·         Curran J.M. Gallagher, J.A. Hunt, J.A. The inflammatory potential of biphasic calcium phosphate granules in osteoblast/macrophage co-culture. Biomaterials, Vol 26 5313-5320. 2005. doi:10.1016/j.biomaterials.2005.01.065 

·         Feugier, P. Black, R.A. Hunt, J.A. How, T.V.  Attachment, morphology and adherence of human endothelial cells to vascular prosthesis materials under the action of shear stress. Biomaterials Vol 26,1457-1466. 2005. doi:10.1016/j.biomaterials.2004.04.050

·         Marques, A. P. Reis, R. L. Hunt, J. A. Cytokine secretion from mononuclear cells cultured in vitro with starch-based polymers and poly-L-lactide. J. Biomed. Materials Res. Vol 71(3), 419-429 2004. doi: 10.1002/jbm.a.30155

·         Jackson,P.V. Hunt, J.A. Doherty, P.J.  Cannon, A.  Gilson, P. Hydrophilicity of 3-D biomaterials: The Washburn equation. Journal of Materials Science: Materials in Medicine, Vol 15, 507-511. 2004. doi: 10.1023/B:JMSM.0000021129.60818.ca

·         Tang, Z.G. Black, R.A.  Curran, J.M. Hunt, J. A. Rhodes, N.P. Williams, D.F. Surface Properties and Biocompatibility of Solvent-cast Poly[ε-caprolactone] Films, Biomaterials Vol 25, 4741-4748. 2004. doi:10.1016/j.biomaterials.2003.12.003 

·         Vallabhaneni SR, Gilling-Smith GL, Brennan JA, Heyes RR, Hunt JA, How TV, Harris PL.  Can monitoring of intrasac pressure accurately predict failure of endovascular aneurysm?  Journal of Endovascular Therapy.  2003. 10:524-530. doi: 10.1583/1545-1550(2003)010<0524:CIPMRP>2.0.CO;2

·         Rice, J.M. Hunt, J.A. Gallagher, J.A. Hanarp, P. Sutherland, D.S. Gold, J. Quantitative assessment of the response of primary derived human osteoblasts and macrophages to a range of nanotopography surfaces in a single culture model in vitro. , Biomaterials, Vol 24, Issue 26, 4799-4818. 2003. doi:10.1016/S0142-9612(03)00381-8 

·         Alves, C.M. Reis, C.M. Hunt, J.A. Preliminary study on human protein adsorption and leukocyte adhesion to starch based biomaterials. J. of Materials Science: Materials in Medicine, Vol 14, 157-165. 2003. doi: 10.1023/A:1022023930825

·         Marques, A.P. Reis, R.L. Hunt, J.A. Evaluation of the potential of starch-based biodegradable polymers in the activation of human inflammatory cells, Journal of Materials Science: Materials in Medicine, Vol 14, 167-173. 2003. doi: 10.1023/A:1022028114896

·         Rice, J.M. Hunt, J.A. Gallagher, J.A. Quantitative evaluation of the biocompatible and osteogenic properties of a range of biphasic calcium phosphate (BCP) granules using primary cultures of human osteoblasts and monocytes. Calcified Tissue International, Vol 72, 6,1. 726-736. 2003.  doi: 10.1007/s00223-002-2045-y

·         Hunt, J.A. and Shoichet M. S.  Biomaterials: drug delivery systems, Current Opinion in Solid State & Materials Science. Vol 6, 281. 2002. doi:10.1016/S1359-0286(02)00115-8 

·         Swindle, E,J, Hunt J.A. Coleman, J.W. A comparison of reactive oxygen species generation by rat peritoneal macrophages and mast cells using the highly sensitive real-time chemiluminescent probe Pholasin; inhibition of antigen-induced mast cell degranulation by macrophage-derived hydrogen peroxide. Journal of Immunology.  Vol 169(10):5866-5873. 2002.

·         Pu, F.R. Williams, R.L. Markkula, T.  Hunt J.A. Expression of Leukocyte-Endothelial Cell Adhesion Molecules on Monocyte Adhesion to Human Endothelial Cells on Plasma Treated PET and PTFE in vitro. Biomaterials Vol 23, 4705-4718 2002. doi:10.1016/S0142-9612(02)00219-3

·         Baldwin, L. Flanagan, B.F. McLaughlin, P.J. Parkinson, R.W. Hunt, J.A. Williams, D.F. A study of interface membranes from revision accord knee arthroplasty: the role of T lymphocytes. Biomaterials Vol 23, 3007-3014. 2002. doi:10.1016/S0142-9612(02)00059-5  

·         Pu, F.R. Williams, R.L. Markkula, T.  Hunt J.A. Effect of plasma treated PET and PTFE on expression of adhesion molecules by human endothelial cells in vitro. Biomaterials Vol 23,2411-2428.  2002. doi:10.1016/S0142-9612(01)00377-5 

·         Marques, A.P. Reis, R.L. Hunt, J.A. The biocompatibility of novel starch based polymers and composites: in vitro studies. Biomaterials. Vol 23, 1471-1478. 2002. doi:10.1016/S0142-9612(01)00272-1

·         Pu, F.R. Williams, R.L. Markkula, T. Hunt, J.A. Monocyte Adhesion and Adhesion Molecule Expression on Human Endothelial cells on Plasma Treated PET and PTFE in Vitro. Materials in Medicine Vol. 12, 971-977. 2001. doi: 10.1023/A:1012861116388

·         Markkula, T.K. Hunt, J.A. Pu, F.R. Williams, R.L. Surface Chemical Derivatisation of Plasma Treated PET and PTFE. Surface and Interface Analysis (SIA),Vol 34, 583-58. 2002. doi: 10.1002/sia.1365

·         Hunt, J.A.  Shoichet, M.  Biomaterials: surface interactions. Current Opinion in Solid State & Materials Science, Vol. 5 Issues 2-3, April - June 161-162. 2001. doi:10.1016/S1359-0286(01)00012-2 

·         Hemingway, R. Williams, R.L. Hunt, J.A. Rudge, S.J. The Influence of Bracket type on the force delivery of Ni-Ti Archwires. European Journal of Orthodontics Vol 23, 233-241. 2001. doi: 10.1093/ejo/23.3.233

·         Briggs. M.C. Greirson, I. Hiscott, P. Hunt, J.A. Active Scatter Factor (HGF/SF) in ProliferativeVitroretinal Disease.  Invest. Opthalmol Vis. Sci. Vol 41:10, September, 3085-3094. 2000.

·         Davey, P.C. Zuzel, M. Kamiguti, A.S. Hunt, J.A. Aziz, K.A. Activation Dependant Proteolytic Degradation of Polymorphonuclear CD11b. British Journal of Haematology.  Vol. 111, 934-942. 2000. doi: 10.1046/j.1365-2141.2000.02401.x

·         Frontiers in biomedical polymer applications. Hunt J.A. Materials Today 1999, 2 (1) 22-23. doi: 10.1016/S1369-7021(99)80036-4 

·         Ramshaw J.A.M. Casagranda, F. White, J.F. Edwards, G.A. Hunt, J.A. Williams, D.F. Werkmeister, J.A.  Effects of Mesh Modification on the Structure of a Mandrel Grown Biosynthetic Vascular Prosthesis.  J. Biomed. Materials Res. Vol. 47, 309-315. 1999. doi: 10.1002/(SICI)1097-4636(19991205)47:3<309::AID-JBM4>3.0.CO;2-R

·         Werkmeister, J.A. Edwards, G.A. White, J.F. Casagranda, F. Hunt, J.A. Williams, D.F. Ramshaw, J.A.M.  In vivo Evaluation of Modified Mandrel Grown Vascular Prostheses. J. Biomed. Materials Res. Vol. 47, 316-323. 1999. doi: 10.1002/(SICI)1097-4636(19991205)47:3<316::AID-JBM5>3.0.CO;2-W

·         Hunt, J.A. Using Computer Based Systems for Biomaterial Evaluation. Editorial, Medical Device Technology, Vol. 10, No 7, 20-23, 1999.

·         Harrington, P.M. Newton, D.J. Williams, C.M.M. Hunt, J.A. Dearman, R.J. Kimber, I. Coleman, J.W. Flanagan, B.F. Eotaxin and eotaxin receptor (CCR3) expression in sephadex particle induced rat lung inflammation. International Journal of Experimental Pathology. Vol. 80, 177-185, 1999. doi: 10.1046/j.1365-2613.1999.00112.x

·         Hunt, J.A. Howlett, C.R. Zicat, B. Williams, D.F. Zreiqat, H.  Quantification of the Bone Related mRNAs at the Bone/Prosthetic Interface. Journal Materials Science: Materials in Medicine, 1998; 9:691-694. doi: 10.1023/A:1008930331180

·         Cartmell, S.H. Doherty, P.J. Hunt, J.A. Healy, D.M. Gilchrist, T. Soft Tissue Response to Glycerol Suspended Controlled Release Glass Particulate.  Journal Materials in Medicine, 1998, 9:773-777. doi: 10.1023/A:1008923523428

·         Rice, J.M. Fisher, A.C. Hunt, J.A. Macrophage Polymer Interactions. Polymer special Cells & Materials Issue J. Biomater. Sci. Polymer Edn.1998; 9: 833-847.

·         Cartmell, S.H. Doherty, P.J. Rhodes, N.P. Hunt, J.A. Healy, D.M. Gilchrist, T.  Haemocompatibility of Controlled Release Glass. Journal Materials Science: Materials in Medicine, 1998; 9:1-7. doi: 10.1023/A:1008830025416

·         Hunt, J. A. McLaughlin, P.J. Flanagan, B.F. Host response to implanted biomaterials: Techniques to investigate cellular and molecular interactions. Biomaterials 1997; 18: 1449-1459. doi:10.1016/S0142-9612(97)00091-4

·         Rhodes, N.P. Hunt, J.A. Williams, D.F. Macrophage subpopulation differentiation by stimulation with biomaterials. Journal of Biomedical Materials Research 1997; 37: 481-488. doi: 10.1002/(SICI)1097-4636(19971215)37:4<481::AID-JBM6>3.0.CO;2-H

·         Hunt JA. Meijs G. Williams D.F. Hydrophillicity of Polymers and Soft Tissue Responses:  A Quantitative Analysis.  Journal of Biomedical Materials Research 1997; 36: 542-549. doi: 10.1002/(SICI)1097-4636(19970915)36:4<542::AID-JBM13>3.0.CO;2-C

·         Hunt JA. Rhodes NP. Williams DF. Flow Cytometry in the Assessment of Biocompatibility. Cellular Engineering 1996; 1: 209-223.

·         Campoccia D. Hunt JA. Doherty PJ. Zhong SP. Oregan M. Benedetti L. Williams DF. Quantitative Assessment of the Tissue-Response to Films of Hyaluronan Derivatives. Biomaterials 1996; 17: 963-975. doi:10.1016/0142-9612(96)84670-9 

·         Hunt JA. Flanagan BF. McLaughlin PJ. Strickland I. Williams DF. The Effect of Biomaterial Surface Charge on the Inflammatory Response: Evaluation of Cellular Infiltration and TNFα Production. Journal of Biomedical Materials Research 1996; 31: 139-144. DOI: doi: 10.1002/(SICI)1097-4636(199605)31:1<139::AID-JBM15>3.0.CO;2-I

·         Gray MR. Darnton SJ. Hunt JA. Irlam RW. Nemeth J. Wallace, HM. Accelerated gastric epithelial proliferation. Gut 1995; 36: 522-527.

·         Hunt JA. Williams DF. Quantifying the Soft Tissue Response to Implanted Materials. Biomaterials 1995; 16:167-170. doi:10.1016/0142-9612(95)92113-K  

·         Hunt JA. Rhodes NP. Williams DF. Analysis of the Inflammatory Exudate Surrounding Implanted Polymers using Flow Cytometry. Journal Materials Science: Materials in Medicine, 1995; 6:839-843. doi: 10.1007/BF00134328

·         Hunt JA. Tavakoli SM. Williams RL. Riches ST.  Laser Surface Modification of Polymers to Improve Biocompatibility.  Journal of Materials Science: Materials In Medicine 1995; 6: 813-817. doi: 10.1007/BF00134323

·         Williams RL. Hunt JA. Tengvall P. Fibroblast Adhesion onto Methyl-Silica Gradients With and Without Preadsorbed Protein. Journal of Biomedical Materials Research, 1995; 29: 1545-1555. doi: 10.1002/jbm.820291211

·         Rhodes NP. Hunt JA. Williams DF.  Quantification of the Host Response to Implanted Polymers in vivo by Flow Cytometry. Journal of Materials Science: Materials in Medicine 1994; 5: 666-670. doi: 10.1007/BF00120353

·         Burthem J. Baker PK. Hunt JA. Cawley JC.  Hairy Cell interactions with extracellular matrix: Expression of specific integrin Receptors and their role in the cell's response to specific adhesive proteins.  Blood 1994; 84: 873-881. http://bloodjournal.hematologylibrary.org/cgi/reprint/84/3/873

·         Burthem J. Baker PK. Hunt JA. Cawley JC.  The Function of c-fms in hairy cell leukaemia: Macrophage Colony Stimulating Factor Stimulates Hairy Cell Movement. Blood 1994; 83: 1381-1389. http://bloodjournal.hematologylibrary.org/cgi/reprint/83/5/1381

·         Hunt JA. Williams DF. Ungersbock A. Perrin S.  The Effect of Titanium Debris on Soft-Tissue Response. Journal of Materials Science: Materials in Medicine 1994; 5: 381-383. doi: 10.1007/BF00058968

·         Ungersbock A. Hunt JA. Comparison of Visual Controlled and Automatic Histomorphometric Evaluation of Soft Tissue. Journal of Materials Science: Materials in Medicine 1994; 5: 702-704. DOI: 10.1007/BF00120360

·         Hunt JA. Abrams KR. Williams DF.  Modelling the Pattern of Cell Distribution Around Implanted Materials.  Analytical Cellular Pathology 1994; 7: 43-52.

·         Hunt JA. Van der Laan JS. Schakenraad J. Williams DF. Quantitative in vivo Assessment of the tissue response to Dermal Sheep Collagen in Abdominal Wall Defects. Biomaterials 1993;14:376-382. doi:10.1016/0142-9612(93)90058-A 

·         Khor E. Hunt JA. Martin PA. Doherty PJ. Williams RL. Williams DF. Non-invasive Magnetic Resonance Imaging of the Soft Tissue Response to a Biomaterial. Clinical Materials 1993; 12: 65-72. doi:10.1016/0267-6605(93)90051-8 

·         Hunt JA. Vince DG. Williams DF. Image-Analysis in the Evaluation of Biomaterials. Journal of Biomedical Engineering 1993; 15: 39-45. doi:10.1016/0141-5425(93)90091-C 

·         Campoccia D. Hunt JA. Doherty PJ. Zhong SP. Callegaro L. Benedetti L. Williams DF.  Human Neutrophil Chemokinesis and Polarisation-Induced by Hyaluronic-Acid Derivatives. Biomaterials 1993; 14: 1135-1139. doi:10.1016/0142-9612(93)90156-V

·         Hunt JA. Remes A. Williams DF. Stimulation of neutrophil movement by metal ions.  Journal of Biomedical Materials Research 1992; 26: 819-828. doi: 10.1002/jbm.820260610

·         Mohanty M. Hunt JA. Doherty PJ. Annis D. Williams DF.  Evaluation of the Soft Tissue Response to a Poly(urethane urea). Biomaterials 1992; 13: 651-656. doi:10.1016/0142-9612(92)90123-6

·         Hunt, JA. Williams, DF. Modification of the soft tissue response to implanted materials through the use of an anti-inflammatory drug.  J. Materials Science: Materials in Medicine 1992; 3: 160-169.  doi: 10.1007/BF00713443

·         Slavin JA. Hunt JA. Nash JR. Kingsnorth AN. Williams DF.  Recombinant basic fibroblast growth factor in red blood cell ghosts accelerates incisional wound healing. Br. J. Surg. 1992; 79: 918-921. doi: 10.1002/bjs.1800790921

·         Hunt JA. Remes A. Williams DF. The effect of metal ions on neutrophil degranulation.  J. Materials Science: Materials in Medicine 1992; 3:.192-198. doi: 10.1007/BF00713449

·         Vince DG. Hunt JA. Williams DF. Quantitative assessment of the tissue response to implanted biomaterials. Biomaterials 1991; 12: 731-736.   doi:10.1016/0142-9612(91)90021-2 

Book Chapters and Other Publications

·         Danoux C. Tare R Smith J Bradley M. Hunt J.A. Oreffo R.  Habibovic P. Materiomics. Chapter 9 Development of materials for regenerative medicine: from clinical need to clinical application. Cambridge University Press. 2013. 151-168.