Sequence based comparative characterization of phages vB_GEC-EfS_2 and vB_GEC-EfS_4
Enterococci common commensal inhabitant of the human gastrointestinal and genitourinary tracts but it can be found as an opportunistic pathogen and cause diseases in nosocomial settings. Multidrug resistant Enterococcus faecalis and Enterococcus faecium infections are especially problematic because they have developed resistance to all available antibiotics, including new drugs tigecycline, linezolid and daptomycin.
In this study we characterized Enterococcus bacteriophages vB_GEC-EfS_2 and vB_GEC-EfS_4. vB_GEC-EfS_2 and vB_GEC-EfS_4 were isolated from the sewage outflow into the River Mtkvari, Tbilisi. Phages were tested against Enterococcus strains from Eliava IBMV and clinical isolates from the Massachusetts Eye and Ear Infirmary (MEEI). Biological properties (phage morphology, host range, growth phenotype, thermal and pH stability) and nucleic acid composition were studied. Whole genomes of phages vB_GEC-EfS_2 and vB_GEC-EfS_4 were sequenced and analyzed. Total length of phage vB_GEC_EFS_2 is 38508bp, The assembly contains 65 ORFs, among them - 3 lysis genes , 13 structural proteins , 1 DNA replication associated, 1 integration , 3 lysis-lysogenic cycle regulation , 43 hypothetical proteins. Phage vB_GEC_EFS_2 is an obligate lysogenic phage (contains 1gene for integration)and proteins shares homologies with E. faecalis specific phage ȹEf11, however unlike phage ȹEf11, vB_GEC_EFS_2 contains holin with haemolysin domain- XhlA and not usable for phage therapy but XhlA gives opportunities for further investigation – in vitro and in vivo experiments to prove haemolysine affect of this phage.
Total length of phage vB_GEC-EfS_4 is 145230bp; the assembly contains ORF-170, promoter-35, terminator-26, and tRNA-12. 99 ORFs proteins share homologies with lytic Enterococcus faecalis phage phiF24C, 99 ORFs covers all important genes: DNA replication, structural and lysis genes. Rest ORFs mostly include hypothetical proteins that are always specific. Phage vB_GEC-EfS_4 exhibit number of properties of potential value for phage therapy.
In this study we characterized Enterococcus bacteriophages vB_GEC-EfS_2 and vB_GEC-EfS_4. vB_GEC-EfS_2 and vB_GEC-EfS_4 were isolated from the sewage outflow into the River Mtkvari, Tbilisi. Phages were tested against Enterococcus strains from Eliava IBMV and clinical isolates from the Massachusetts Eye and Ear Infirmary (MEEI). Biological properties (phage morphology, host range, growth phenotype, thermal and pH stability) and nucleic acid composition were studied. Whole genomes of phages vB_GEC-EfS_2 and vB_GEC-EfS_4 were sequenced and analyzed. Total length of phage vB_GEC_EFS_2 is 38508bp, The assembly contains 65 ORFs, among them - 3 lysis genes , 13 structural proteins , 1 DNA replication associated, 1 integration , 3 lysis-lysogenic cycle regulation , 43 hypothetical proteins. Phage vB_GEC_EFS_2 is an obligate lysogenic phage (contains 1gene for integration)and proteins shares homologies with E. faecalis specific phage ȹEf11, however unlike phage ȹEf11, vB_GEC_EFS_2 contains holin with haemolysin domain- XhlA and not usable for phage therapy but XhlA gives opportunities for further investigation – in vitro and in vivo experiments to prove haemolysine affect of this phage.
Total length of phage vB_GEC-EfS_4 is 145230bp; the assembly contains ORF-170, promoter-35, terminator-26, and tRNA-12. 99 ORFs proteins share homologies with lytic Enterococcus faecalis phage phiF24C, 99 ORFs covers all important genes: DNA replication, structural and lysis genes. Rest ORFs mostly include hypothetical proteins that are always specific. Phage vB_GEC-EfS_4 exhibit number of properties of potential value for phage therapy.
Reference:
Posters Day 2-T03-Pos-12
Session:
Posters Covering Ecology, Host population control, Co-Evolutionary dynamics and Subversion/Evasion of Host Defences
Presenters:
Sophio Rigvava
Session:
Day 2 Posters Covering: Ecology, Host population control, Co-evolutionary dynamics and Subversion/Evasion of host defences
Presentation type:
Poster presentation
Room:
Poster Halls
Date:
Tuesday, 19 July 2016
Time:
12:05 - 15:00