Piobacteriophage is one of complex phage cocktails, which are produced by the Russian pharmaceutical company Microgen. A metagenomic analysis of the composition of piobacteriophage cocktail was carried out, taxonomic identification and reference mapping revealed T7 supergroup phages as the most numerous fraction of this cocktail. The obtained sequences possessed a high degree of similarity with sequences of K. pneumonia bacteriophages (unclassified Podoviridae, and genera phiKMVlikevirus, T7likevirus); P. aeruginosa bacteriophages (phiKMVlikevirus and Luz24likevirus); Staphylococcus phages (family Myoviridae, genus Twortlikevirus), Proteus phages (genus phiKMVlikevirus), and E. coli phages (Myoviridae, genus T4likevirus). Bioinformatic analysis did not reveal undesired genes and sequences of temperate bacteriophages.
Clinical strains of pathogenic bacteria isolated out of diabetic foot ulcers were tested for sensitivity to the piobacteriophage cocktail and if the bacteria were phage sensitive, treatment by piobacteriophage was applied. Local applications of piobacteriophage were used in eight cases of patients with staphylococcal infection (3 MRSA cases) and in three cases of patients with P. aeruginosa infection. Bacteria were absent after 5-th day phage exposure in six cases of staph infection and in all cases of infection of P. aeruginosa.