The application of bacteriophage to combat bacterial infections is a promising alternative to conventional antibiotics. Nevertheless, phage resistance can be observed after application. In order to circumvent resistance development, a combination of different phages may be utilized. These phage cocktails could hinder the rise of phage insensitive bacteria and subsequently prolong the therapeutic effect of the therapy. Hence, an optimal combination of phages is crucial for the success of the treatment. Favourably phages with overlapping host ranges recognizing different host surface receptors as target can be combined. We therefore established an easy to handle high throughput screening assay to identify phage receptors. A Tn5 transposon mutant library of the plant pathogen Erwinia amylovora, the causative agent of fire blight, was constructed. A collection of strictly lytic Erwinia specific phages belonging to the family of Myo- and Podoviridae were examined. The assay was successfully adapted for phage types T7, Felix01, Sp6, Vi1, an unassigned N4 and Y2 phages. Library screening was performed using 96-well soft agar plates. Mutants revealing a phage resistant phenotype were validated with classical soft-agar overlay and spot on the lawn assays. Furthermore, the transposon insertion site was identified for validated mutants using a two-step arbitrary PCR. This method allowed quick identification of genes involved in phage resistance. Aside from the identification of phage receptors on the host surface, this assay helps elucidate the special predator prey relationship of phage and its host.