Expanding Our Representation of the Diversity of Coliphages


Kema Malki1, 2, Andrea Garretto2, Alexandria Cooper2, Katherine Bruder1, 2, Catherine Putonti2

1University of South Florida, Saint Petersburg, United States
2Loyola University Chicago, Chicago, United States


Escherichia coli persists in a variety of forms, including commensal as well as highly virulent strains. The input of pathogenic strains into the environment (e.g. soil, freshwaters, etc.) is thus closely monitored. These enteric bacteria are however susceptible to predation, most notably by coliphages, which can also be found in these different environments. To date almost 100 coliphage genomes have been sequenced, varying in their genome size, organization and content. In order to further develop our knowledge of these phages and the roles they play, it is essential that we continue to isolate and sequence phage genomes. I have isolated seven novel, originally lysogenic coliphages. Characterization of these phages included a study of burst time and generation time. While visualization through TEM indicates similar morphotypes, growth characteristics vary between individual strains. Here we present the characterization of these phages as well as complete genome sequences.






Reference:
Posters Day 2-T03-Pos-66
Session:
Posters Covering Ecology, Host population control, Co-Evolutionary dynamics and Subversion/Evasion of Host Defences
Presenters:
Kema Malki
Session:
Day 2 Posters Covering: Ecology, Host population control, Co-evolutionary dynamics and Subversion/Evasion of host defences
Presentation type:
Poster presentation
Room:
Poster Halls
Date:
Tuesday, 19 July 2016
Time:
12:05 - 15:00