from MRC
cDNA sequence:
agcgtggggctcggttccctggctccggccgcggggggagcatggagttt
gcggagctgattaagaccccgcgggtggacaatgtggtgctgcaccggcc
tttctacccggctgtcgagggcaccctgtgcctgacgggccaccacttga
tcctgtcctcccggcaggacaatacggaggagctgtggctcctccattca
aacatcgacgccatcgacaagcgatttgtaggatcactgggtaccatcat
cataaaatgtaaagattttcgaattattcagttggatattcctggaatgg
aggaatgcttgaatatagccagttccattgaggcattgtctactctggac
tccatcactctgatgtaccctttcttttaccgtcctatgtttgaagtgat
agaagatggctggcattccttccttcctgagcaagaatttgaactctatt
cttcagctaccagtgaatggaggctaagctatgtcaataaggaatttgct
gtctgtccctcttacccaccaattgtcacagtgcccaaatccatcgatga
tgaagctcttcggaaggtagctacatttcgacatggagggcgcttcccag
tactcagctattaccacaaaaaaaatgggatggtaattatgcgaagtggt
cagccactcactggtacaaacgggaggaggtgcaaggaggacgagaagct
gataaatgctaccctcagggctggaaagcgtggctacatcattgacaccc
gatccctgaacgtggctcagcaaactagagccaaaggaggtggctttgaa
caagaagctcattatcctcagtggaggcgaattcataagtccattgagag
gtatcacattcttcaggagagcttaatcaaacttgtggaagcttgtaatg
accaaacacataacatggaccgatggctcagtaaattggaggcctctaac
tggctgactcacatcaaagagattctgacaactgcctgcctagcggctca
gtgcatcgacagggaaggagcatcaatattgattcacggaacagaaggaa
ctgattccacactccaggtgacctccttggcccagatcatcttagagcca
agaagcaggaccattcgtggttttgaggccctgattgaaagagagtggct
gcaggctggtcacccattccagcagcgctgtgcactgtcagcctactgta
acaccaagcagaagtgggaggctcctgtatttcttctcttcttggactgc
gtgtggcagatccttcgtcagtttccctgttcttttgagtttaatgagaa
tttcctcatcatgctctttgagcatgcttatgcctcacagtttggaacat
ttctgggcaacaatgaaagtgaaagatgtaagttgaagctacagcagaag
acgatgtctttgtggtcctgggttaatcagcccagtgagctgagtaaatt
caccaatcccctctttgaagccaacaaccttgtcatctggccttcagttg
ctccgcagagtcttccactgtgggaaggtattttcctacgttggaataga
tcctctaagtatttggatgaagcatatgaagaaatggttaacatcattga
atataataaagaattacaagcaaaagtcaatatccttcgaaggcagttgg
cagaactggaaacagaggacgggatgcaggagagtccctgaaaggtctcc
tcgcacccttcgcaaggaccttcttgggcctgtgtccgccgttctctcct
tgtgcccttcagttcacttttacacggtagccttgaagtgaaggctttag
atgtgggacccccctaatgtaatggatttctcaatactgtatgaataatg
aaacttactatcataaaccatgtttcatgtggcctgttagggcctgtcac
tttgggagccggaaggaggtgctagtcattttatttttacgtgaaataga
tgacctatttaatgccaaaaaaaaaaaaaaaa
Protein Sequence (549 aa):
mefaeliktprvdnvvlhrpfypavegtlcltghhlilssrqdnteelwl
lhsnidaidkrfvgslgtiiikckdfriiqldipgmeeclniassieals
tldsitlmypffyrpmfeviedgwhsflpeqefelyssatsewrlsyvnk
efavcpsyppivtvpksiddealrkvatfrhggrfpvlsyyhkkngmvim
rsgqpltgtngrrckedeklinatlragkrgyiidtrslnvaqqtrakgg
gfeqeahypqwrrihksieryhilqesliklveacndqthnmdrwlskle
asnwlthikeilttaclaaqcidregasilihgtegtdstlqvtslaqii
leprsrtirgfealierewlqaghpfqqrcaqsaycntkqkweapvfllf
ldcvwqilrqfpcsfefnenflimlfehayasqfgtflgnnesercklkl
qqktmslwswvnqpselskftnplfeannlviwpsvapqslplwegiflr
wnrsskyldeayeemvniieynkelqakvnilrrqlaeletedgmqesp
SUBCLONING STRATEGY FOR YEAST TWO-HYBRID SCREENING AND CO-IP/CO-IF