from MRC
cDNA sequence:
ggcggctagaccggtggcggcggcatctggagtagcactgagatcttccg
gtggcgggaactgcagtcatggatcatattacggtacccaaggtagaaaa
cgtgaaattggtggatcgttatgtgagtaagaaaccagctaatgggattc
tttatcttactgcaacccacctgatctatgtggaggcttcaggtgcagcc
cggaaagaaacatggattgcactccatcacattgccactgtggagaagtt
acccatcactagcctgggttgtcccctgaccctccgctgcaagaatttcc
gggtggcccactttgttttagattctgaccttgtgtgccatgaggtttat
atttcactgctcaagctttctcagccagcattacctgaagatctttatgc
tttttcttataatcccaaatcctcaaaagagatgagggaaagtggatgga
aactgattgacccaatatcagactttgggcgtatgggaatacccaacaga
aactggaccataacagatgccaacagaaactatgagatatgcagcaccta
ccctcctgaaatagtggttcctaaatctgttaccttgggaacggtggttg
gaagttcaaagttcagaagtaaagaacgtgtccctgtgctctcctacctc
tacaaagagaacaatgctgccatttgccgctgtagccagcctctctctgg
attttacactcgctgtgtagatgatgagctcttgttggaggccattagcc
aaacaaacccagggagccagtttatgtatgttgtagacacaagaccaaag
ttgaatgccatggccaaccgagcagctgggaaggggtatgaaaatgaaga
caactatgccaacattcgcttcagattcatgggcattgagaacatccatg
taatgcggagcagtctgcagaaactcttggaagtttgtgaattgaaaact
ccaacaatgagtgaatttcttagcggcctggagagctcagggtggttaag
acacattaaagctattatggatgctggaattttcattacaaaggcagtga
aggtagaaaaggccagtgtcttagtccattgttctgatggatgggaccgc
acagcacaagtctgctcagtggctagcatcctcctagatccattttatag
gacattcaaaggactcatgatcttgatagagaaggaatggatatccatgg
gccacaagttttcccaaaggtgtggccacctcgatggggactctaaagaa
gtgtcccctatcttcacccagttcctagactgtatctggcaattaatgga
acagtttccctgtgcctttgagtttaatgaaaacttcctgctggagattc
atgaccatgttttctcctgccagtttggaaacttccttggtaactgccag
aaggatcgggaagatctaagagtctatgagaaaacacattctgtgtggcc
tttcttggttcagaggaaaccagacttcaggaaccctctctataaaggct
tcactatgtatggggtactcaatcctagtactgtgccctacaacattcag
ttctggtgtgggatgtataaccgctttgacaaagggctgcagcccaagca
gagtatgctagagagcctcctggaaattaagaaacagagagcaatgctgg
agacagatgtgcatgaactagaaaagaaactaaaagtccgtgatgagcca
ccagaagagatctgtacctgctctcaattaggaaacatattatcccagca
tctgggaagtcctttgaccaatcctcttggctttatgggtatcaatggag
acctgaataccctgatggagaatggcaccctatccagggaaggaggcctc
cgagctcagatggatcaagtaaaaagccagggtgcagacctccaccataa
ctgttgtgagattgtgggcagccttagggccataaatatctctggggatg
tgggcatctctgaggccatgggcatctctggagacatgtgcacctttgag
gctacgggcttctccaaagacttaggaatctgtggggccatggacatctc
tgaggccactggcatctctggaaacttgggtatttctgaggccaggggtt
tctctggggacatgggcatcttgggggacacaggcatctccaaggccagc
accaaggaggcagactactccaagcatcagtgagttagttactcatcttc
catgctaggtatacctacagcagctacaatgattcttgtcccagagaggg
ctatgctgcttttccaattggtttaagtgggtgaagccagggcacccctc
tccccacttaagtctggaagggaattactttactgattaagtgaccaata
tgttcatattaagcagtacagagtgtaggcaagagaaagaatatgttatt
atattaagcagtacagagtgtaggcaagagaaagaatatgttatt
gttctaaaatgggatttaactatagaaatgttgcctctcagctgagacac
atattaagctggttctactgtctgttttccttaattatcgacctgcccta
ttttctatcctcactgcagtaatgtgaaagtgaagtggaaatattgtcac
aatggtcaataatgacccttttgagggtatggagagatggtatacagcag
gtaactgtgaattgcagaataaattgaaagaaaattttaaaaaaaaaaaa
aaa
Protein Sequence (704 aa):
mdhitvpkvenvklvdryvskkpangilyltathliyveasgaarketwi
alhhiatveklpitslgcpltlrcknfrvahfvldsdlvchevyisllkl
sqpalpedlyafsynpksskemresgwklidpisdfgrmgipnrnwtitd
anrnyeicstyppeivvpksvtlgtvvgsskfrskervpvlsylykenna
aicrcsqplsgfytrcvddellleaisqtnpgsqfmyvvdtrpklnaman
raagkgyenednyanirfrfmgienihvmrsslqkllevcelktptmsef
lsglessgwlrhikaimdagifitkavkvekasvlvhcsdgwdrtaqvcs
vasilldpfyrtfkglmiliekewismghkfsqrcghldgdskevspift
qfldciwqlmeqfpcafefnenflleihdhvfscqfgnflgncqkdredl
rvyekthsvwpflvqrkpdfrnplykgftmygvlnpstvpyniqfwcgmy
nrfdkglqpkqsmleslleikkqramletdvhelekklkvrdeppeeict
csqlgnilsqhlgspltnplgfmgingdlntlmengtlsregglraqmdq
vksqgadlhhncceivgslrainisgdvgiseamgisgdmctfeatgfsk
dlgicgamdiseatgisgnlgiseargfsgdmgilgdtgiskastkeady
skhq
SUBCLONING STRATEGY FOR YEAST TWO-HYBRID SCREENING AND CO-IP/CO-IF