from Nandurkar's lab
cDNA sequence:
atgggcggagccccagccgctagtgctggtggccgccgctgctgccccag
ctggagcccgagccgccgcctctgcgtccgcgcgtcgctgcttcccaggg
cggcggtatgctggggaaaggagtagtcggcggtggcggcggcaccaagg
cccccaagccctccttcgtgtcgtacgtacgccctgaggaaattcacaca
aacgaaaaggaagtaacagagaaggaagtaactcttcacttgttgccagg
tgaacagctgctttgtgaagccagcacagtactgaagtatgtccaggaag
attcctgtcagcatggggtctatgggaggcttgtctgcacagacttcaag
attgccttcttgggtgatgatgaatctgcattggataatgatgaaactca
atttaagaataaggttataggagaaaatgacattacactccactgtgttg
atcagatttatggagtgtttgatgagaaaaagaaaactctctttggacaa
ctgaagaaataccctgagaagctcatcatccactgcaaagaccttcgagt
gttccagttttgtctgaggtacacaaaggaagaggaagtcaaaaggattg
tcagtggcataattcatcatacccaggctcctaaactgcttaaacgatta
tttctgttttcctatgcgactgctgcacaaaacaatacagtcactgatcc
caagaaccataccgtaatgtttgacacacttaaggactggtgttgggaac
tggaacggaccaaaggcaacatgaagtacaaagcagtgagtgtcaacgaa
ggctataaagtctgtgagagattgccagcatactttgttgtccccacccc
tcttcctgaagagaatgtgcagcgctttcagggtcatggcataccaatat
ggtgttggtcctgccacaatggaagtgcccttttgaaaatgtcagcactg
cccaaagaacaggatgacggcattttacaaatccaaaagagcttcttaga
tggaatttacaagaccatccacaggccaccctatgaaattgttaaaacgg
aagacctgtcaagcaacttcctgtccctgcaggaaatccagactgcatac
tctaaatttaaacagctatttctgatagataacagtactgaattttggga
cacagatataaaatggttttctctgttggaaagtagcagctggcttgaca
taatcagacgttgcctgaaaaaagcaatagagattacagaatgtatggaa
gcacaaaacatgaatgttcttcttttagaggagaatgcatccgacctctg
ctgtctcatttcctctctggtgcaactgatgatggacccccactgcagaa
ccagaattggtttccagagcctcatccaaaaggagtgggtcatgggtggc
cactgtttcttggatcgctgcaaccatctccgccagaacgacaaagagga
ggttcctgtgttcctgcttttcctagattgtgtctggcagctggtgcacc
agcatcccccggcatttgaattcacagagacttacctgactgttttgtca
gatagcctgtatatacctatttttagcaccttcttcttcaattcacctca
tcaaaaagatactaacatgggtagagaaggccaggatacacaaagcaagc
ctttgaatctgctcaccgtgtgggattggtcggtgcagtttgaacccaaa
gcccagacattgctcaaaaaccctctttatgtggaaaagccaaaactgga
caaaggccaacggaaaggaatgcgcttcaaacatcaacgacaactttctt
tgccacttacacaatctaagtcatctcccaaaagaggatttttcagggaa
gaaacagatcatttaattaaaaaccttatgggcaagagaattagcaaact
tattaattcttctgatgagctccaagacaactttcgagagttctatgaca
gctggcatagcaagtccactgactaccatggtttgttgttaccgcatatc
gaggggcccgaaatcaaagtctgggcccagcgctacctacgttggattcc
agaagcccaaatcctaggtggtggccaagtggccactctgagcaaactct
tggaaatgatggaggaagtccagagtttacaagagaagatcgacgagaga
caccacagccagcaggccccccaggctgaggccccctgcctgctgaggaa
ctctgcccgcctctcgtctttgtttcctttcgctctgctccagcgacatt
cctctaagcccgtcttacccaccagtggctggaaagctttgggagatgaa
gacgatttggccaaacgagaagatgagttcgtggacctaggggatgtgtg
acctgtttgttgagtgattgtgaaagaggaatgtgggagattgggacagc
tgatccctggatttgtgtcacgctacagctcatgctggctgggtccttag
cccggcactccgttagaataaacctgggccttcgggaaataaaaaaaaaa
aaaaaaaaaaaa
Protein Sequence (747 aa):
Note: Original MTMR12 from Nandurkar's lab had already two aa mutated (Met591 to Leu, Trp639 to Arg)
MLGKGVVGGGGGTKAPKPSFVSYVRPEEIHTNEKEVTEKEVTLHLLPGEQ
LLCEASTVLKYVQEDSCQHGVYGRLVCTDFKIAFLGDDESALDNDETQFK
NKVIGENDITLHCVDQIYGVFDEKKKTLFGQLKKYPEKLIIHCKDLRVFQ
FCLRYTKEEEVKRIVSGIIHHTQAPKLLKRLFLFSYATAAQNNTVTDPKN
HTVMFDTLKDWCWELERTKGNMKYKAVSVNEGYKVCERLPAYFVVPTPLP
EENVQRFQGHGIPIWCWSCHNGSALLKMSALPKEQDDGILQIQKSFLDGI
YKTIHRPPYEIVKTEDLSSNFLSLQEIQTAYSKFKQLFLIDNSTEFWDTD
IKWFSLLESSSWLDIIRRCLKKAIEITECMEAQNMNVLLLEENASDLCCL
ISSLVQLMMDPHCRTRIGFQSLIQKEWVMGGHCFLDRCNHLRQNDKEEVP
VFLLFLDCVWQLVHQHPPAFEFTETYLTVLSDSLYIPIFSTFFFNSPHQK
DTNMGREGQDTQSKPLNLLTVWDWSVQFEPKAQTLLKNPLYVEKPKLDKG
QRKGMRFKHQRQLSLPLTQSKSSPKRGFFREETDHLIKNLMGKRISKLIN
SSDELQDNFREFYDSWHSKSTDYHGLLLPHIEGPEIKVWAQRYLRWIPEA
QILGGGQVATLSKLLEMMEEVQSLQEKIDERHHSQQAPQAEAPCLLRNSA
RLSSLFPFALLQRHSSKPVLPTSGWKALGDEDDLAKREDEFVDLGDV
SUBCLONING STRATEGY FOR YEAST TWO-HYBRID SCREENING AND CO-IP/CO-IF