Born 1966. B.Sc. (Hons) Zoology with Marine Zoology (UCNW Bangor UK, 1988); Ph.D. Physiology (Liverpool UK, 1991); SEDA Cert. in External Examining (Keele UK, 2010).
1985-88: BSc Honours degree in Zoology with Marine Zoology. Honours project (Dave Grove’s lab, Ocean Sciences, UCNW Bangor). My scientific career in gastrointestinal physiology began in 1997-8, investigating the endocrine regulation of upper gastrointestinal function in fish, using ex vivo organ strips and in vivo studies to examine gallbladder contraction and gastric emptying.
1988-91. As a research assistant in Liverpool, I gained my PhD in Physiology (Nov. 1991) in laboratory of Graham Dockray FRS (Physiology Laboratory, Liverpool), investigating the endocrine control of avian gastric and pancreatic function. This work involved in vivo models, feeding studies, endocrine cell isolation and culture; resulting in five full peer-review publications:
- Key publications: Biochim. Biophys. Acta 1990;1048;66-71; Am J Physiol. 1991; 261;G16-G21; Eur. J. Pharmacol. 1991; 209; 231-235; Reg. Peptides 1994;49;249-255; Eur. J. Biochem. 1995;230;439-446.
1991-94: As MRC-funded post-doctoral fellow (working in Jon Rhodes’ lab, Dept. of Medicine, Liverpool). I developed key methods, knowledge and skills in epithelial glycobiology, investigating mucus secretory cell and mucin glycoprotiens. Studies focused on the identification of oncofetal carbohydrate structures on mucins in colorectal cancer and inflammatory bowel disease, using high-pH, high performance chromatography techniques.
- Key publications: Biomed. Chromatogr. 1993;7,68-74; J. Chromatogr. 1993;622,137-146; J. Clin. Invest. 1995;95,571-76.
1994-5: During a temporary teaching lectureship, held for 1 year as part of the Wellcome Trust-funded Leave fellowship awarded to Rod Dimaline (Physiology, Liverpool), I used molecular biology approaches to examine the regulation of GATA transcription factors in the gastrointestinal epithelium.
- Key publication: Gastroenterology 1997;112,1559-1567.
1995-2003: Appointed to a full-time academic position (Medicine, Liverpool), my independent research led to specific understanding of causes and consequences of the altered mucosal glycosylation that occurs in colorectal inflammation and neoplasia. Pioneering observations demonstated changes in mucosal carbohydrate structures resulting from altered Golgi acidification and action of cytokines. Techniques including 2-D and ex vivo tissue explants culture. I was also member of the EU Concerted Action consortium ‘Mucins in the pathophysiology of inflammatory diseases of the bronchial & gastrointestinal epithelia’ (1998-2001).
- Key publications: Methods Mol Biol 1998;76,161-182; Glycobiology 2001,11,385-93; Glycoconjugate J. 2001,18,851-8; Trends Mol. Med. 2002,8,10-16; Glycobiology 2006;16:594-601.
2003-2008: Appointed to Senior Lecturer (Clinical Science, Liverpool) research focused on examination of how aberrant glycosylation could drive altered recruitment of mucosa-associated adherent bacteria seen in increased numbers in patients with Crohn’s disease and colon cancer, in particular Adherent, Invasive E. coli (AIEC). Established as one of the key international teams leading research into the host mechanisms within epithelial cells and macrophages relevant to interaction with these E. coli in Crohn’s disease (and more recently in colitis-associated colorectal cancer).
- Key Publications: Gastroenterology 2004; 127, 80-93; Gastroenterology 2007; 133:1487-98; Inflam. Bowel Dis. 2008; 14:162-75; Antimicrob Agents Chemother 2008; 52:427-34; Microbiology 2008; 154:571-83;
2009-2013: Appointment as Reader in Gastroenterology (Institute of Translational Medicine, Liverpool; 2009).
- Key Publications: Nature Medicine 2010; 16:90-7; Gut 2010; 59:1331-9; Gut 2010 60: 26-33; J. Bacteriol. 2011; 193:4540-1; J.Clin.Invest. 2011; 121:966-75; Gut 2011; 60: 26-33; Science 2012; 338: 120-3.
2013: Promotion to Full Professor - Personal Chair in Gastrointestinal Physiology & Translational Medicine (Liverpool; Oct 2013).
2013-2020: Professor in Molecular & Cellular Physiology (Institute of Translational Medicine; Liverpool; Oct 2013- May 2020).
- Key Publications: J. Nutri. Biochem. 2013, 24:97-103; Disease Models & Mechanisms 2013; 6:1388-99; PLoS ONE 2014, 9(2): e87658; Inflammatory Bowel Diseases 2014; 20(11):1919-32; Gut 2014, 63: 761–770; Inflam Bowel Dis. 2015; 21(7):1499-510; Aliment Pharmacol Ther. 2015, 42(2):158-79; Scand J Gastroenterol. 2018, 53(6):677-684; PLoSONE 2018, 13(10):e0202460; Cell Tiss. Res. 2019, 375:409–424; Scientific Reports 2019, 9: 193; Pathogens 2019, 8: 74; Scientific Reports 2019, 9: 9328; Frontiers in Cellular & Infection Microbiology 2019, 9:30; Frontiers in Immunology 2019; 10: 2168; Nutrition Bulletin 2019, 44: 329; Cell Death & disease 2019, 10: 896.
2020-present: Professor in Infection Biology & Microbiomes (Institute of Infection, Veterinary & Ecological Sciences; Liverpool; May 2020).
- Key Publications: Aliment.Pharmacol.Therapeut. 2020; 51(12), 1268-1285; Dis Model Mech. 2020, 13(11):dmm044040; Cell Biochem Funct. 2021, 39(3):401-412; Curr. Pharmaceut. Design. 2021, doi: 10.2174/1381612827666210218141847; Int J Mol Sci. 2021, 22(7):3646; Frontiers in Immunology 2021 OPEN ACCESS; Gut Microbes 2021, 13(1) OPEN ACCESS;
The Campbell lab (in collaboration with key international groups from France and Sweden), has furthered understanding of the interaction, internalization and translocation of AIEC across microfold (M)-cells of the follicle-associated epithelium (FAE) overlying Peyer’s patches, which is dependent on possession of long polar fimbriae (Lpf). A new award from Crohn’s & Colitis UK will identify the receptor for Lpf. Adhesion between the intestinal epithelium and mucosa-associated E. coli (and various enteric gut pathogens) can also be prevented by soluble dietary plant fibres. This led to commercial partner interest with Provexis Plc to develop the therapeutic potential of soluble plantain fibre in the treatment of Crohn’s disease and infective diarrhoeas (BBSRC LINK and CASE awards to examine the role of dietary fibre in blockade of Salmonellae, ETEC and C. difficile). With funding from NIHR we have also evaluated new therapeutic approaches targeting Crohn’s associated AIEC recruitment to the inflamed mucosa (epithelial & M cells) and those replicating within the macrophage phagolysosome. Research examining intramacrophage killing of AIEC with a combination of antibiotics and hydoxychloroquine has led to a clinical trial with this treatment regimen to facilitate remission of patients with active disease. We have also been providing key insight into the mechanisms through which certain bacteria inhabitant of the intestinal microbiota could have an implication in the pathogenesis of intestinal inflammation and colorectal cancer (CRC). In collaboration with the Jobin lab UNC Chapel Hill (USA), AIEC, influenced by intestinal inflammation, appear to drive CRC progression through direct genotoxin (colibactin)-induced epithelial DNA damage. Support from NWCR, will assess if E. coli found more commonly to be interacting with the inflamed and cancerous tissue of bowel can support an environment for a transformed/pre-cancerous cell in the bowel to flourish (e.g. oncogenic NFkappaB activation, Wnt pathway activation, escaping apoptosis, promotion of cell growth and angiogenesis). Identifying which strains are deleterious and determining the specific microbial signature(s) of these strains, investigating whether carriage of specific E. coli strains is a risk factor in CRC onset and progression, and how potential ‘genotoxic’ bacteria contribute to the time-course of tumour process in bowel, will be the subject of proposals to AICR, NIH and MRC. Discovery of novel actions for microbes in the development of CRC may lead to translational interventions to modulate microbial composition and prevent tumour development, including prebiotic, probiotic and ‘contrabiotic’ (dietary supplementation with soluble plant fibre) strategies.
We are also using state-of-art imaging of the inflamed and infected intestinal epithelium in vivo and in 3-D organotypic ‘mini guts’ and 3-D organoids.
We have already shown that using high resolution in vivo confocal endomicroscopy it is possible to quantify bacteria on the surface and within the inflamed human intestinal epithelium. We have recently been characterizing, using confocal microscopy, epithelial cell shedding (and gap formation) in intestinal epithelia in vivo following inflammatory stimuli and will move to examination of how members of the NF-kappaB family of transcription factors regulate this process in mouse models of IBD; also whether bacteria can gain entry into these gaps. Our aim is also to be in a position to obtain detailed ultrastructural analysis of key bacterial interactions with specific gut cells using high-resolution 3D-EM coupled with the 3View system.
Impact of dietary exposure to emulsifiers on the intestinal mucosa - implications for inflammatory bowel disease and metabolic syndrome (2017-2021; UKRI/MRC).
Bacterial folate synthesis and long-term health (2017-2021; 4-year BBSRC DTP Studentship; with Durham University).
Understanding how the intestinal epithelium regulates intestinal barrier function to prevent intestinal disease and systemic inflammation (2021-2024; LIBYAN EMBASSY IN LONDON Studentship)
Study of adherent, invasive Escherichia coli receptor interactions in the intestinal epithelium.(2021-2024); LIBYAN EMBASSY IN LONDON Studentship)
Use of enteroid cultures as platforms for the discovery of compounds with anti-cryptosporidial activity (2021-2024); China Scholarship Council/UoL Studentship).
Research Network affliations
Microbiome Innovation Research Centre
Regenerative Medicine Network
Liverpool Cancer Research Institute
Institute of Infection, Veterinary & Ecologicial Sciences
Wellcome Trust 4 year PhD programme in Cellular & Molecular Physiology (1994-2023)
SysmedIBD EU-FP7 consortium (2012-2017)
European Network for Gastro-Intestinal Health Research 2010-2014
The Gut Microbiota for Health Expert Panel (GMfH panel)
The GMfH comes under the auspices of the British Society of Gastroenterology (BSG) and is a multi-disciplinary group that includes primary and secondary care clinicians (including gastroenterologists), microbiologists, dietitians and research scientists, all of whom have expertise and interest in the gut microbiota and its role in health and disease. Since 2013, the GMfH panel has made contributions to the development of guidelines for faecal microbiota transplantation (FMT), written major papers reviewing gut microbiota research, has run BSG workshops and contributed to the KTN Microbiome Innovation Network report.4
BSG (2019) The Ecologist will see you now
KTN (2021) Microbiome Innovation Network microbiome strategic roadmap report (May 2021)
Guts UK (2021) Full gut microbiome (Poo) testing leaflet
BSG (2021) Gut microbiome testing leaflet (Short version)
Linda Thomas/GMfH (2021). Complete Nutrition Vol.21 • No.4 • June 2021 Commercial Gut Microbiome Testing
Technical approaches used by this laboratory
- Bacteria-host interactions: Adhesion, invasion, M cell translocation and cell signalling assays (incl. kinases and G-proteins).
- Bacterial adhesin-glycoconjugate interactions.
- In vitro Gastrointestinal epithelial cell culture and modelling: Organoid and organotypic culture. M-cell culture and 2-D transwell cultures.
- Immunohistochemistry and molecular biological techniques
- Nutrient-epithelium actions
- In vivo confocal/multi-photon microscopy.
- TEM and 3View electron microscopy.
- Ex vivo metabolic studies on isolated intestinal tissue explants.
- Protein and glycoprotein purification (FPLC, ion exchange and affinity chromatography).
- Mucus barrier analysis, mucus glycoprotein purification and MUC gene expression (including real-time PCR)
- O-linked and N-linked oligosaccharide analysis (HPLC, HP-TLC and HPAEC-PAD).
- Lectin-carbohydrate interactions.
United European Gastroenterology Week (UEGW) Oral free paper prize (2011)
The Simms lecture 2016 - Royal College of Physcians Annual Update in Medicine conference, York Racecourse; Dec 2016
UK State of the Art Lecture 2018 - British Infection Association - London; 17 May 2018
Peer review of tenders for the Scottish Government’s Strategic Research Programme (SRP) in Environment, Natural Resources and Agriculture (2022-7). Sept 2021.
European Helicobacter & Microbiota Study Group - 2020 Committee member (Virtual conference)
British Society of Gastroenterology (London) - BSG Campus 2020 - Programme committee member (2019-2020) Virtual conference
Anatara LifeSciences Ltd (Australia) - Product Development Scientific Advisory Board (2018-present)
UK Gut Microbiota for Health Expert Panel (2013-present)
British Society of Gastroenterology (London) Science in Gastroenterology lead (2014-present)
British Society of Gastroenterology (London) Research Committee member (2014-present) - BSG Research Committee
British Society of Gastroenterology (London) Education Committee member (2014-present) - BSG Education Committee
British Society of Gastroenterology (London) Sir Francis Avery Jones medal award selection panel (2016-present)
British Society of Gastroenterology (London) UEG Rising stars nomination panel (2016-present)
Health Research Board (HRB) - Patient-Oriented Research grant selection panel; Dublin, Ireland (2013)
SysmedIBD (EU-FP7) Steering Committee; Koln, Germany (2013)
Scientists in Gastroenterology Committee; British Society of Gastroenterology; London, UK (2012)
Euroglycoscience Forum - Education Committee (2007-2010)
External examiner appointments
External Examiner - MSc Molecular Medicine - University of Sheffield (2021-2022)
External Examiner - Year 1 & 2 MBBS at St Georges Medical School London (2019-2020)
External Examiner - Year 1 & 2 MBBS at St Georges Medical School London (2018-2019)
External Examiner - Year 1 & 2 MBBS at St Georges Medical School London (2017-2018)
External Examiner - Year 1 & 2 MBBS at St Georges Medical School London (2016-2017)
External Examiner - Year 1 & 2 MBBS at St Georges Medical School London (2015-2016)
External Examiner - Year 2 MBChB at Keele University Medical School(2011-2012)
External Examiner - Year 2 MBChB at Keele University Medical School(2010-2011)
External Examiner - Year 2 MBChB at Keele University Medical School(2009-2010)
External Examiner - Year 2 MBChB at Keele University Medical School(2008-2009)
External higher degree examiner appointments
External PhD Examination - Monash University, Melbourne, Australia (2020)
External PhD Examination - University of Strathclyde, UK (2017)
External PhD Examination - University of Strathclyde, UK (2016)
External PhD Examination - University of Glasgow, UK (2016)
External PhD Examination - University of Manchester, UK (2015)
External PhD Examination - University of Lancaster, UK (2014)
External PhD Examination - University of Aberdeen, UK (2013)
External PhD Examination - University College Cork, Eire (2012)
External teaching/assessment appointments
External Lecturer/assessment - MSc Nutritional Medicine, University of Surrey (2019)
External Lecturer/assessment - MSc Nutritional Medicine, University of Surrey (2017)
External Lecturer/assessment - MSc Nutritional Medicine, University of Surrey (2015)
External Lecturer/assessment - MSc Nutritional Medicine, University of Surrey (2013)
External Lecturer/assessment - MSc Nutritional Medicine, University of Surrey (2011)
BSG Annual meeting; Glasgow, UK; June 2019 - Translational Science Masterclass: ‘Faecal microbiota transplantation – from bench to bedside’
BSG Annual meeting; Liverpool, UK; June 2018 - Translational Science Masterclass: ‘Diet, digestion, health & disease’;
BSG Annual meeting; Manchester, UK; June 2017 - Translational Science Masterclass:‘Epithelial signalling in inflammation and cancer’;
BSG Annual meeting; Liverpool, UK; June 2016 - Translational Science Masterclass: ‘Gut enteroendocrine system’
Digestive Diseases Federation [ACPGBI/AUGIS/BAPEN/BASL & BSG] Annual meeting; London Excel, June 2015 - Translational Masterclass: ‘Gut microbiome in health and disease’
BSG Annual meeting; Manchester, UK; June 2014 Translational Science Masterclass: ‘Gut and liver inflammation’
Teaching and Administration
- Sir Alastair Pilkington Award for Sustained Excellence in Teaching (Liverpool 2002)
- SEDA Professional certificate in External Examining; (Keele University 2010)
School of Medicine
- Year 1 MBChB - Gastrointestinal system module lead (2014-present)
- Year 2 MBChB - Gastroenterology module lead (2015-present)
- School of Medicine Board of Studies
- Board of Examiners Year 1 MBChB
- Board of Examiners Year 2 MBChB
- ‘Standards setting’ Boards for Year 1 and Year 2
- University Academic Advisor - Year 1 and Year 2 MBChB students
Teaching Year 1 MBChB - Gastrointestinal System block lead:
Teaching Year 2 MBChB: - Gastroenterology module Non-clinical lead:
- Gastroenterology module lecture: Lecture -'Alcohol's trip through the body'
- Foundation block 3 module lecture: Lecture -'Inflammation & Cancer'
- Case-based learning (CBL) - 2 tutor groups
- Review Lectures (x 3)- Learning outcomes, CBL, Block Assessment
- Graduate entry review tutorial session 1 (2h; Y1 GI Lectures 1-8)
- Graduate entry review tutorial session 2 (2h; Y1 GI Lectures 12-16)
School of Dentistry
Teaching Year 1 Dentistry BDS:
Other Teaching material: Key Concepts in Digestion
Institute of Translational Medicine
- Institute representative on Institute of Learning & Teaching School of Medicine Board of Studies
- Director of Postgraduate Research for Department of Cellular & Molecular Physiology (PhD/MD/MPhil)
- Member of ITM Postgraduate Research Committee (2012-present)
- Academic mentor for Faculty Higher Degree students (MPhil/MD/PhD)
- Master in Research (MRes) in Biomedical Sciences programme - 'Physiology' & 'Gastroenterology' strands; Frontiers in Research lecture & Journal Club and Research Projects.
- 4 year PhD programme in Cellular & Molecular Physiology Frontiers in Research lecture & Journal Club and Research Projects.
And now for something different....
Frau A, Ijaz UZ, Slater R, Jonkers D, Penders J, Campbell BJ, Kenny JG, Hall N, Lenzi L, Burkitt MD, Pierik M, Darby AC, Probert CSJ (2021). Inter-kingdom relationships in Crohn’s disease explored using a multi-omics approach. Gut Microbes 13(1), DOI: 10.1080/19490976.2021.1930871 OPEN ACCESS
Papoutsopoulou S, Pollock L, Walker C, Tench W, Samad SS, Bergey F, Lenzi L, Sheibani-Tezerji R, Rosenstiel P, Alam MT, Martins Dos Santos VAP, Müller WJP and Campbell BJ (2021). Impact of interleukin 10 deficiency on intestinal epithelium responses to inflammatory signals. Frontiers in Immunology. Epub 16 June 2021 OPEN ACCESS
Mahalhal A, Burkitt MD, Duckworth CA, Hold GL, Campbell BJ, Pritchard DM, Probert CS (2021). Long-Term Iron Deficiency and Dietary Iron Excess Exacerbate Acute Dextran Sodium Sulphate-Induced Colitis and Are Associated with Significant Dysbiosis. Int J Mol Sci. 22(7):3646. OPEN ACCESS
Papoutsopoulou S, Campbell BJ (2021). Epigenetic modifications of the nuclear nactor kappa B signalling pathway and its impact on inflammatory bowel disease. Current Pharmaceutical Design. doi: 10.2174/1381612827666210218141847. Online ahead of print. PMID: 33602081
Jagadeesh N, Belur S, Campbell BJ, Inamdar SR (2021). The fucose-specific lectin ANL from Aspergillus niger possesses anti-cancer activity by inducing the intrinsic apoptosis pathway in hepatocellular and colon cancer cells. Cell Biochemistry & Function 2021; 1–12. AUTHOR SHARE.
Lloyd K, Papoutsopoulou S, Smith E, Stegmaier P, Bergey F, Morris L, Kittner M, England H, Spiller D, White MHR, Duckworth CA, Campbell BJ, Poroikov V, Martins Dos Santos VAP, Kel A, Muller W, Pritchard DM, Probert C, Burkitt MD; SysmedIBD Consortium (2020). Using systems medicine to identify a therapeutic agent with potential for repurposing in inflammatory bowel disease. Dis Model Mech. 2020 Nov 27;13(11): dmm044040 OPEN ACCESS
Papoutsopoulou S, Satsangi J, Campbell BJ, Probert CS (2020). Review article: impact of cigarette smoking on intestinal inflammation—direct and indirect mechanisms. Alimentary Pharmacology & Therapeutics; 51(12), 1268-1285 OPEN ACCESS .
Duckworth CA, Jones L, Thompson L, Vaida A, Caamano J, Burkitt M, Miyajima F, Williams J, Ikuomola F, Campbell BJ, Pritchard DM (2019). NF-κB2 signalling in enteroids modulates enterocyte responses to secreted factors from bone marrow-derived dendritic cells. Cell death & Disease 10, 896 OPEN ACCESS .
Partridge D, Lloyd KA, Rhodes JM, Walker AW, Johnstone AM, Campbell BJ (2019). Food additives: Assessing the impact of exposure to permitted emulsifiers on bowel and metabolic health – introducing the FADiets study. Nutrition Bulletin 44, 329-349 OPEN ACCESS
Papoutsopoulou S, Burkitt MD, Bergey F, England H, Hough R, Schmidt L, Spiller DG, White MRH, Paszek P, Jackson DA, Martins dos Santos VAP, Sellge G, Pritchard DM, Campbell BJ, Muller W and Probert CS (2019). Macrophage-specific NF-kB activation dynamics can segregate inflammatory bowel disease patients.
Frontiers in Immunology 10, 2168 OPEN ACCESS
Luu L, Johnston LJ, Derricott H, Armstrong S, Randle N, Hartley C, Duckworth C, Campbell B, Wastling J, Coombes JL (2019). An open-format enteroid culture system for interrogation of interactions between Toxoplasma gondii and the intestinal epithelium. Frontiers in Cellular and Infection Microbiology 9, 300 OPEN ACCESS
Frau A, Kenny J, Lenzi L, Campbell BJ, Ijaz U, Duckworth CA, Burkitt M, Hall N, Anson J, Darby A, Probert CS (2019). DNA extraction and amplicon production strategies deeply influence the outcome of gut mycobiome studies. Scientific Reports 9: 9328 OPEN ACCESS
Tawfik A, Knight P, Duckworth CA, Pritchard DM, Rhodes JM, Campbell BJ (2019). Replication of Crohn’s Disease Mucosal E. coli Isolates inside Macrophages Correlates with Resistance to Superoxide and Is Dependent on Macrophage NF-kappa B Activation. Pathogens 8, 74 OPEN ACCESS
Minshawi F, White M, Muller W, Humphreys N, Jackson D, Campbell BJ, Adamson A, Papoutsopoulou S. (2019) Human TNF-Luc reporter mouse: A new model to quantify inflammatory responses. Scientific Reports 9: 193 OPEN ACCESS
Derricott H, Luu L, Fong WY, Armstrong SD., Randle N, Duckworth CA, Campbell BJ, Wastling J, Coombes JL. (2019). Developing a 3D intestinal epithelium model for livestock species. Cell and Tissue Research 375:409–424 Full text view only version
Mahalhal A, Williams JM, Johnson S, Ellaby N, Duckworth CA, Burkitt MD, Liu X, Hold GL, Campbell BJ, Pritchard DM, Probert CS (2018). Oral iron exacerbates colitis and influences the intestinal microbiome. PlosONE 13(10):e0202460. OPEN ACCESS
Yakymenko O, Schoultz I, Gullberg E, Ström M, Almer S, Wallon C, Wang A, Keita ÅV, Campbell BJ, McKay DM, Söderholm JD (2018). Scand J Gastroenterol. 53(6):677-684. OPEN ACCESS
Merga YJ, O'Hara A, Burkitt MD, Duckworth CA, Probert CS, Campbell BJ, Pritchard DM (2016). Am J Physiol Gastrointest Liver Physiol. 310(11):G1081-90. OPEN ACCESS
Simpson HL, Campbell BJ (2015). Review article: dietary fibre–microbiota interactions. Aliment Pharmacol Therapeut. 42(2):158-79 OPEN ACCESS
Flanagan PK, Chiewchengchol D, Wright HL, Edwards SE, Alswied A, Satsangi J, Subramanian S, Rhodes JM, Campbell BJ (2015). Killing of Escherichia coli by Crohn’s disease monocyte-derived macrophages and its enhancement by hydroxychloroquine and Vitamin D. Inflamm. Bowel Dis. 21(7):1499-510.
Parsons BN, Campbell BJ, Wigley P (2015). Soluble plantain nonstarch polysaccharides, although increasing caecal load, reduce systemic invasion of Salmonella Gallinarum in the chicken. Lett Appl Microbiol. 60(4):347-51
Simpson HL, Campbell BJ, Rhodes JM (2014). IBD: microbiota manipulation through diet and modified bacteria. Dig Dis. 32, Suppl 1:18-25
Williams JM, Duckworth CD, Burkitt MD, Watson AJM, Campbell BJ, Pritchard DM (2014). Epithelial Cell Shedding and Barrier Function: A Matter of Life and Death at the Small Intestinal Villus Tip. Vet Pathol. 52(3):445-55
Dogan B, Suzuki H, Herlekar D, Sartor RB, Campbell BJ, Roberts CL, Stewart K, Scherl EJ, Araz Y, Bitar PP, Lefébure T, Chandler B, Schukken YH, Stanhope M, Simpson KW. (2014). Inflammation-associated adherent-invasive E. coli are enriched in pathways for use of propanediol and iron, and M cell translocation. Inflammatory Bowel Diseases. 20(11):1919-32.
Rasheed F, Campbell BJ(*Corresponding author), Alfizah H, Varro A, Zahra R, Yamaoka Y, Pritchard DM (2014). Analysis of Clinical Isolates of Helicobacter pylori in Pakistan Reveals High Degrees of Pathogenicity and High Frequencies of Antibiotic Resistance. Helicobacter 19: 387–399
Tawfik A, Flanagan PK, Campbell BJ (2014). Escherichia coli-host macrophage interactions in the pathogenesis of inflammatory bowel disease. World J Gastroenterol 2014; 20(27): 8751-8763
Parsons BN, Wigley P, Simpson HL, Williams JM, Humphrey S, et al. (2014) Dietary Supplementation with Soluble Plantain Non-Starch Polysaccharides Inhibits Intestinal Invasion of Salmonella Typhimurium in the Chicken. PLoS ONE 9(2): e87658. OPEN ACCESS
Merga Y, Campbell BJ, Rhodes JM (2014). Mucosal barrier, bacteria and IBD: possibilities for therapy. Digestive Diseases 32(4):475-83
Simpson HL, Campbell BJ (2014). 'Soluble dietary plant non-starch polysaccharides may improve health by inhibiting adhesion, invasion and translocation of enteric gut pathogens. In, 'Polysaccharides: Natural Fibers in Food and Nutrition'. Published: 30th June 2014 by CRC Press; Editor: Benkeblia, N Invited contribution
Prorok-Hamon M, Friswell MK, Alswied A, Roberts CL, Song F, Flanagan PK, Knight P, Codling C, Marchesi JR, Winstanley C, Hall N, Rhodes JM, Campbell BJ (2014). Colonic mucosa-associated diffusely adherent afaC+ Escherichia coli expressing lpfA and pks are increased in inflammatory bowel disease and colon cancer. Gut 2014; 63: 761–770. OPEN ACCESS
Williams JM, Duckworth CA, Watson AJM, Frey MR, Miguel JC, Burkitt MD, Sutton R, Hughes KR, Hall LJ, Caamaño J, Campbell BJ, Pritchard DM (2013). A Mouse Model of Pathological Small Intestinal Epithelial Cell Apoptosis and Shedding Induced by Lipopolysaccharide. Disease Models & Mechanisms. 6:1388-1399.OPEN ACCESS
Roberts CL, Keita ÅV, Parsons BN, Prorok-Hamon M, Knight P, Friswell MK, Winstanley C, O’Kennedy N, Söderholm JD, Rhodes JM, Campbell BJ (2013). Soluble plantain fibre blocks epithelial adhesion and M-cell translocation of intestinal pathogens. J Nutritional Biochem 24(1): 97–103. OPEN ACCESS
Arthur JC, Perez-Chanona E, Mühlbauer M, Tomkovich S, Uronis JM, Fan T-J, Campbell BJ, Abujamel T, Dogan B, Rogers AB, Rhodes JM, Stintzi A, Simpson KW, Hansen JJ, Keku TO, Fodor AA, Jobin C (2012) 'Intestinal inflammation targets cancer-inducing activity of the microbiota'. Science 338, 120-123
Flanagan P, Campbell BJ, Rhodes JM (2012). Lessons from diversion studies and antibacterial interventions. Digestive Diseases 30(4)347-50
Flanagan P, Campbell BJ, Rhodes JM (2011). Bacteria in the pathogenesis of inflammatory bowel disease. Biochem Soc Trans 39(4): 1067-72
Flanagan P, Campbell BJ, Rhodes JM (2011). Host-microbiota interactions in inflammatory bowel disease - Bacteria, good & bad. Biochemist 33(4): 22-25
Chassiang B, Rolhion N, de Vallée A, Salim SY, Prorok-Hamon M, Neut C, Campbell BJ, Söderholm JD, Hugot J-P, Colombel J-F, Darfeuille-Michaud A (2011). Crohn’s disease-associated adherent-invasive Escherichia coli target murine and human Peyer’s patches via Long Polar Fimbriae. J Clin Invest. 121: 966–975
Clarke DJ, Chaudhuri RR, Martin HM, Campbell BJ, Rhodes JM, Constantinidou C, Pallen MJ, Loman NJ, Cunningham AF, Browning DF, Henderson IR (2011). Complete genome sequence of the Crohn's disease-associated adherent-invasive Escherichia coli strain HM605. J. Bacteriol. 193(17): 4540-1
Flanagan P, Campbell BJ, Rhodes JM (2011). Bacteria in the pathogenesis of inflammatory bowel disease. Biochem Soc Trans 39(4): 1067-72
Stewart RMK, Wiehlmann L, Ashelford KE, Preston SJ, Frimmersdorf E, Campbell BJ, Neal TJ, Hall N, Tuft S, Kaye SB, Winstanley C (2011). Genetic characterization indicates a specific sub-population of Pseudomonas aeruginosa associated with keratitis infections. J. Clin. Microbiol. 49: 993-1003
Moussata D, Goetz M, Gloeckner A, Kerner M, Campbell BJ, Hoffman A, Biesterfeld S, Flourie B, Saurin J-C, Galle PR, Neurath MF, Watson AJM, Kiesslich R (2010).Confocal laser endomicroscopy is a new imaging modality for recognition of intramucosal bacteria in inflammatory bowel disease in vivo. Gut 60: 26-33
Roberts CL, Keita AV, Duncan SH, O'Kennedy N, Söderholm JD, Rhodes JM, Campbell BJ (2010).Translocation of Crohn’s disease E. coli across M-cells: contrasting effects of soluble plant fibres and emulsifiers. Gut 59: 1331-1339
Rhodes JM, Campbell BJ, Yu L-G (2010). Glycosylation and Disease. In, Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: Chichester. DOI:10.1002/9780470015902.a0002151.pub2
Gaynor JW, Campbell BJ, Cosstick R. (2010). RNA interference: a chemist's perspective. Chem Soc Rev. 2010 39(11):4169-84
Friswell M, Campbell BJ, Rhodes JM (2010). The role of bacteria in the pathogenesis of inflammatory bowel disease. Gut Liver 4: 295-306
Cooney R, Baker J, Brain O, Danis B, Pichulik T, Allan P, Ferguson DJ, Campbell BJ, Jewell D, Simmons A. (2010) NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation. Nature Medicine 16: 90-7
Subramanian S, Roberts CL, Hart CA, Martin HM, Edwards SW, Rhodes JM, Campbell BJ (2008). Replication of colonic Crohn's disease mucosal E. coli isolates within macrophages and their susceptibility to antibiotics. Antimicrob. Agents Chemother. 52: 427-4340
Knight P, Campbell BJ, Rhodes JM (2008). Bacteria-host interactions in inflammatory bowel disease. British Medical Bulletin. 88: 95-113
Rhodes JM, Campbell BJ, Yu L-G (2008). Lectin-epithelial interactions in the human colon. Biochem Soc Trans. 36: 1482-6
Gaynor J, Fisher J, Campbell B, Cosstick R (2008). Incorporation of 3'-S-phosphorothiolates into RNA: potential applications in RNAi. Nucleic Acids Symp. 52: 319-20
Subramanian S, Rhodes JM, Hart CA, Tam B, Roberts CL, Smith SL, Corkill JE, Winstanley C, Virji M and Campbell BJ (2008). Characterisation of epithelial IL-8 response to inflammatory bowel disease mucosal E. coli and its inhibition by mesalamine. Inflammatory Bowel diseases 14: 162-175
Bronowski C, Smith SL, Yokota K, Corkill JE, Martin HM, Campbell BJ, Rhodes JM, Hart CA, Winstanley C (2008). A subset of mucosa-associated Escherichia coli isolates from patients with colon cancer, but not Crohn’s disease, share pathogenicity islands with urinary pathogenic E. coli. Microbiology 154: 571-83
Tralau T, Vuilleumier S, Thibault C, Campbell BJ, Hart CA, Kertesz MA (2007). Transcriptomic analysis of the sulfate starvation response of Pseudomonas aeruginosa. J. Bacteriol. 189: 6743-50
Campbell BJ, Yu LG, Rhodes JM (2007). Mucosal glycoconjugates in inflammatory bowel disease and colon cancer. In, Glycobiology. Sansom C, Markman O (eds). Scion Publishing Ltd. pp 261-273.
CM Mpofu, BJ Campbell, S Subramanian, S Marshall-Clarke, CA Hart, A Cross, CL Roberts, A McGoldrick, SW Edwards, JM Rhodes (2007). Microbial Mannan Inhibits Bacterial Killing By Macrophages: A Possible Pathogenic Mechanism for Crohn’S Disease. Gastroenterology 133: 1487-98
Subramanian S, Campbell BJ, Rhodes JM (2006). Bacteria in the pathogenesis of inflammatory bowel disease. Curr Opin Infect Dis. 19: 475-84
Singh R, Subramanian S, Rhodes JM, Campbell BJ (2006). Peanut lectin stimulates proliferation of colon cancer cells by interaction with glycosylated CD44v6 isoforms and consequential activation of c-Met and MAPK: functional implications for disease-associated glycosylation changes. Glycobiology 16: 594-601.
Martin HM, Campbell BJ, Hart CA, Mpofu CM, Nayar M, Singh R, Englyst H, Williams HF and Rhodes JM (2004). Enhanced Escherichia coli Adherence and Invasion in Crohn’s Disease and Colon Cancer. Gastroenterology 127: 80–93
Campbell BJ, Yu LG, Rhodes JM (2001). Altered glycosylation in inflammatory bowel disease: A possible role in cancer development. Glycoconjugate Journal 18 (11-12): 851-858 (2003 publication)
Yu LG, Andrews N, Weldon M, Gerasimenko OV, Campbell BJ, Singh R, Grierson I, Petersen OH, Rhodes JM (2002). An N-terminal truncated form of Orp150 is a cytoplasmic ligand for the anti-proliferative mushroom Agaricus bisporus lectin and is required for nuclear localisation sequence-dependent nuclear protein import. J. Biol Chem 277: 24538-24545.
Rhodes JM, Campbell BJ (2002). Inflammation and colorectal cancer: IBD associated and sporadic cancer compared. TRENDS in Molecular Medicine 8: 10-16.
Leiper K, Campbell BJ, Jenkinson MD, Milton JM, Yu LG, Democratis J, Rhodes JM (2001). Interaction between bacterial peptides, neutrophils and goblet cells: a possible mechanism for neutrophil recruitment and goblet cell depletion in colitis. Clinical Science 101: 395–402
Singh R, Campbell BJ, Yu LG, Fernig DG, Milton JD, Goodlad RA, FitzGerald AJ, Rhodes JM (2001). Cell surface-expressed Thomsen-Friedenreich antigen in colon cancer is predominantly carried on high molecular weight splice variants of CD44. Glycobiology 11: 587-592.
Campbell BJ, Rowe GE, Leiper K, Rhodes JM (2001). Increasing the intra-Golgi pH of cultured LS174T goblet-differentiated cells mimics the decreased mucin sulfation and increased Thomsen-Friedenreich antigen (Galbeta1-3GalNacalpha-) expression seen in colon cancer. Glycobiology 11: 385-93.
Campbell BJ, Leiper K, Rowe G, Rhodes JM (2000). “Exploration of mechanisms which determine mucosal sulphation in the healthy and diseased human colon”, in Digestive Mucus: From Research to Clinical Implications (Eds. J Bara, Y Christen, MT Droy-Lefaix). Edition IRVINN, Paris. pp 37-40
Campbell BJ (1999). "Biochemical and functional aspects of mucus and mucin-type glycoproteins", in Bioadhesive Drug Delivery Systems: Fundamentals, Novel approaches and Development. (Eds. E Mathiowitz, DE Chickering III and C-M Lehr). Marcel Dekker, Inc., New York. Chapter 5, pp 85-130
Pereira SP, Rhodes JM, Campbell BJ, Kumar D, Bain IM, Murphy GM, Dowling RH (1998). Biliary lactoferrin concentrations are increased in active inflammatory bowel disease: a factor in the pathogenesis of primary sclerosing cholangitis? Clin Sci (Lond) 95:637-44
Campbell BJ, Rhodes JM (1998). Purification of gastrointestinal mucins and analysis of their O-linked oligosaccharides. Methods Mol Biol. 76: 161-82
Campbell BJ, Rhodes JM (1998). "Metabolic studies on isolated intestinal tissue", in Methods in Disease: Investigating the gastrointestinal tract. (Eds. V Preedy & R Watson), Greenwich Medical Media Ltd., London. Chapter 14, pp 157-168
Dimaline R, Campbell BJ, Watson F, Sandvik AK, Struthers J, Noble PJ (1997). Regulated expression of GATA-6 transcription factor in gastric endocrine cells. Gastroenterology 112: 1559-67
Stretch GL, Campbell BJ, Dwarakanath AD, Yaqoob M, Stevenson A, Morris AI, Rhodes JM (1996). 5-amino salicylic acid absorption and metabolism in ulcerative colitis patients receiving maintenance sulphasalazine, olsalazine or mesalazine. Aliment Pharmacol Ther. 10: 941-7
Finnie IA, Campbell BJ, Taylor BA, Milton JD, Sadek SK, Yu LG, Rhodes JM (1996). Stimulation of colonic mucin synthesis by corticosteroids and nicotine. Clin Sci (Lond) 91 : 359-64
Rhodes JM, Campbell BJ, Evans RC (1996) "Mucosal defences"; in, Inflammatory Bowel Disease, 3rd Edition (Ed. RN Allan et al.,) Churchill-Livingstone, Edinburgh: pp 143-155
Dwarakanath AD, Campbell BJ, Tsai HH, Sunderland D, Hart CA, Rhodes JM (1995). Faecal mucinase activity assessed in inflammatory bowel disease using 14C threonine labelled mucin substrate. Gut 37: 58-62
Wu SV, Walsh JH, Campbell BJ, Dimaline R (1995).Molecular characterization and physiological regulation of a TATA-less gene encoding chicken gastrin. Eur J Biochem. 230: 439-46
Campbell BJ, Finnie IA, Hounsell EF, Rhodes JM (1995). Direct demonstration of increased expression of Thomsen-Friedenreich (TF) antigen in colonic adenocarcinoma and ulcerative colitis mucin and its concealment in normal mucin.J Clin Invest. 95: 571-6
Rhodes JM, Campbell BJ, Finnie IA (1994). "Mucus and the gastrointestinal tract", in Recent Advances in Gastroenterology 10 (Ed. R Pounder) Churchill-Livingstone, Edinburgh; Chapter pp 57-79
Campbell BJ, Garner A, Dockray GJ, Hughes J, Dimaline R (1994). The mechanism of action of gastrin-releasing peptide in stimulating avian gastric acid secretion. Reg. Peptides 49; 249-255
Campbell BJ, Davies M, Rhodes JM, Hounsell EF (1993). Separation of neutral oligosaccharide alditols from human meconium using high pH anion exchange chromatography. J. Chromatogr. (B) 622; 137-146
Parker N, Raouf AH, Finnie IA, Ryder SD, Campbell BJ, Tsai HH, Iddon D, Milton JD, Rhodes JM (1993). High performance gel filtration using monodisperse highly cross-linked agarose as a one step system for mucin purification. Biomedical Chromatography 7; 68-74
Campbell BJ, Dimaline R, Dockray GJ, Hughes J (1991). Inhibition of food intake by omeprazole in the chicken. Eur. J. Pharmacol. 209; 231-235
Campbell BJ, Garner A, Dimaline R, Dockray GJ (1991). Hormonal control of the avian pancreas by gastrin-releasing peptide from proventriculus. Am. J. Physiology 261; G16-21
Campbell, B.J. The endocrine control of avian upper gastrointestinal function. (PhD thesis; University of Liverpool, 1991)
Campbell BJ, Young J, Dimaline R, Dockray GJ (1990). Isolation, sequence and biosynthetic significance of a novel fragment of gastrin-releasing peptide from chicken proventriculus. Biochim. Biophys. Acta 1048; 66-71
Scientific commentaries on my research
REF2014 Impact Case study Improved Inflammatory Bowel Disease Treatment by Reducing Unsafe Corticosteroid Use
A reliable mouse model for studying intestinal epithelial damage. Dis. Model. Mech. Nov.2013, 6 (6), p1299
Vetrano S, Danese S (2013). Colitis, Microbiota, and Colon Cancer: An Infernal Triangle. Gastroenterology. 144(2), 461-463.
Linking Inflammation to Bacteria and Cancer. AICR ScienceNow 43 Winter 2013, pp1-2 (www.aicr.org )
Williamson R (2012). News & Views: Genes carried by E.coli bacteria linked to colon cancer. Colorectal Cancer 1(5), 366.
Kåhrström CT (2012). Bacterial pathogenesis: E. coli claims the driving seat for cancer. Nature Reviews Microbiology 10, 670-671 (October 2012) | doi:10.1038/nrmicro2878.
Genes carried by E.coli bacteria linked to colon cancer
Schwabe RF, Wang TC. Bacteria Deliver a Genotoxic Hit. Science - 5 October 2012: Vol. 338 no. 6103 pp. 52-53. DOI: 10.1126/science.1229905.
Cancer-Causing Gut Bacteria (The Scientist) - 17 August 2012 read more
E. coli strain linked to cancer in mice (Nature | News) - 16 August 2012 read more
A banana a day keeps the Salmonella at bay - American Society of Microbiology (18 June, 2012) web site
Greenhill C (2011). Crohn's disease: Adherent-invasive Escherichia coli target Peyer's patches. Nat Rev Gastroenterol Hepatol. 8(5):246.
Lawrance IC (2011). NOD2 and CD. Inflammatory Bowel Diseases 17(12); 2596–2597
Strober W (2011). Adherent-invasive E. coli in Crohn’s disease: bacterial “agent provocateur”. J Clin Invest. 121(3):841–844
Giles C (2010). Can plant fibres help treat Crohn’s? Welcome News Issue 65, p10
Franks I (2010). Plant soluble fibers may protect against E. coli translocation. Nature Reviews in Gastroenterology & Hepatology 7(12); 2 (Dec 2010).
Shin JN, Eissa NT (2010). Autophagy gets the ‘NOD’ to enhance bacterial handling and antigen presentation. Immunology and Cell Biology 88, 343–345.
Netea MG, Joosten LA (2010). A NOD for autophagy. Nature Medicine 2010; 16(1), 28-30.
Tack J, Carethers JM (2007). Mannan as a trigger for Crohn’s disease through suppression of macrophage function. Gastroenterology 133: 1398-1399
Rioux K, Dieleman L (2005). Mucosal adherence of Escherichia coli in inflammatory bowel disease: Learning more about our closest “friends”. Inflammatory Bowel Diseases 11 (1), 76-77.
Media & Patient group engagement
Organoids - the future of medical research
Bowel cancer risk and E. coli bacteria
Crohn's and Colitis UK Clinical Community interview with Amanda Barrell: Clinical communities manager http://ccuk.clinicalcommunities.net/ (6 Aug 2013)
Bowel cancer ‘could be fuelled by E. coli stomach bug’ (The Daily Mail) – 20 August 2012
Colitis & Crohn’s UK (2011). Research news. NACC Newsletter No. 67, p3.
Colitis & Crohn’s UK (2011). Research news. NACC Newsletter No. 65, p5 & 9.
Colitis & Crohn’s UK (2010). Research news. NACC Newsletter No. 64, p9.
Briggs H (2010). Broccoli 'boosts' healthy gut. BBC News (26th Aug 2010).
Adams S (2010). Broccoli and plantain could fight Crohn's disease. The Telegraph (26th Aug 2010)
Anon. (2010). Broccoli could help treat Crohn's disease by halting bacteria. The Daily Mail (26th Aug 2010)
Key note speaker - NWCRF Committee meeting, Liverpool CRUK Cancer Research Centre. 5th May 2010.
Cockcroft L (2007). Hope of a successful treatment for Crohn’s disease. 11 Dec 2007. The Telegraph. http://www.telegraph.co.uk/news/uknews/1572178/Researchers-hopeful-of-Crohns-treatment.html
Talk at Liverpool Medical Institute, Liverpool, UK. Nov 2007
Invited talk to the NACC at Ysbyty Gwynedd, Bangor, North Wales. 31st July 2007.
National Association for Colitis & Crohn’s disease (1999). Gut mucus. NACC Newsletter No. 39
R & D news (RLBU NHS Trust). Sticky E. coli, bananas and Crohn’s disease. (Issue 1, Oct 2004)
Invited talk at the Roy Castle International Cancer Centre. Liverpool. Jan 2003.