| We
moved in the new labs in Nicholson Building in summer
2003. From left: The Finningan TSQ7000 GC/LC-MS in GC
mode, the Agilent 6890N GC, the Agilent 100 Series HPLC
and the CEInstruments CHN analyser. These are the main
workhorses of the group although they often used by other
working groups. |
The labs of the OBG group are
well equipped with a wide range of apparatus and sophisticated
machinery. Wet preparation organic chemistry is catered for
with numerous extraction, distillation and filtration glassware,
fume cupboards, ovens, rotary evaporators, two muffle furnaces,
one freeze dryer and four centrifuges amongst other things.
One of the centrifuges is a microcentrifuge (Jouan, MR23i) temperature
controlled system. There is also a tangential flow filtration
unit (Easy Load Masterflex - Millipore). On the analytical side
the most important equipment are:
- One GC/LC-MSMS (TSQ-7000). This machine
is a sophisticated and modern piece of equipment and it is
used mainly for research. It was bought from Finnigan-Thermoquest
and it can function both in LC and GC mode as well as MS and
MSMS modes. It is equiped with a HPLC quarternary pump (Spectra
System P400) and a autosampler (AS100) when operating in LCMS/MS
mode (either in ACPI or ESI mode) and with a TRACE 2000 Series
GC when operating in GSMS/MS mode. The instrument is attached
to a NT Workstation equipped with Excalibur software for further
processing.
- Six GCs (together with the Marine Pollution
group). One Agilent 6890N (with FID, ECD and TCD detectors),
four HP5890 (FID and ECD detectors) and one Carlo Erba 5300
Mega Series (wih FID detector) .
- Three HPLCs. One Agilent 1100 Series equipped
with a quartenary pump and a diode array and flourescence
detectors, a ternary SP8800 pump with a flourescence detector
and one equipped with a binary Spectra Series P200 pump with
a SP8450 UV/VIS detector.
- One CHN analyser (NC 2500 - CE Instruments)
- Various spectrophotomenters used both by
our group, the rest of the research groups as well as undergraduate
students in the department.
Gas Chromatography is one of the techniques
most commonly used by our group and when coupled with mass spectrometry
(GCMS) is used to separate individual compounds (for example
lipids) isolated from environmental samples. Many organic compounds
can not be analysed by GCMS, because they are too big and have
very high boiling point or too polar or ionised. These are normally
separated using liguid chromatography. A pump is used to "push"
the liquid phase through a chromatographic column. This is High
Performance Liquid Chromatography (HPLC) and commonly a flouorimeter
or a UV detector is connected at the end of the column. When
an HPLC system is connected to a mass spectrometer, this is
(HP)Liquid Chromatography Mass Spectrometry (HPLCMS). In the
Organic Biogeochemistry Group, HPLCMS is used for the analysis
and identification of pigments, lipid conjugates, proteins and
peptides.
|