2 <- 1,2,3 for number of codons to be analysed; set to 4 if require 3 codon dhfr genotype omitting 'impossible' clones 8 <- level of precision required for ML estimate 3 <- level of precision required for CI estimation 12 <- maximum number of clones in any sample n <- (must be y or n) whether 'minority' genotypes will be missed in typing 0.3 <-the detection limit if minority genotypes are missed e.g. 0.3 means genotypes present at frequency less than 30% will be missed… y <- (must be y or n) whether MOI is known for each sample 1 <- distribution type to be used if MOI is unknown n <- (must be y or n) whether to check hillclimbing always converges on the same ML 'peak' n <- (must be y or n) whether to check programme accuracy by simulating datasets and checking 95% of estimates fall within the 95% CI H <- (must be H or L in uppercase) If a dataset is simulated should it be for a High or Low transmission setting? 100 <- required size of dataset for simulations to check programme accuracy 1000 <- number of replicates used to check hillclimbing or programme accuracy 0 <- a redundant parameter, set to zero. [This allows later programme versions to acquire additional information without making previous input files incompatible] 0 <- a redundant parameter, set to zero. 0 <- a redundant parameter, set to zero. 0 <- a redundant parameter, set to zero 0 <- a redundant parameter, set to zero.